致病性嗜水气单胞菌PCR快速检测方法的建立

Abstract

Abstract: PCR method to detect 16S rDNA gene and aerolysin gene of Aeromonas hydrophila was established, in which two pairs of primers according to the conserved 16S rDNA gene and aerolysin gene sequence of Aeromonas hydrophila were used. 12 strains were tested for pathogenic A.hydrophila by PCR, the results showed that all the 12 strains of Aeromonas hydrophila contain 16S rDNA gene, and 3 strains contain Aerolysin gene. BLAST analysis showed that the amplified sequences were coincident with the published 16S rDNA gene and Aerolysin gene of Aeromonas hydrophila on GenBank.

Key words: Aeromonas hydrophila; 16S rDNA; Aero gene

CLC Number:

ZHU Zhi-xiu, DENG Shun-zhou, YANG Zhu-qing, WU Xiang-dong, WAN Gen, ZHANG Wen-bo, LUO Hong-liang. Establishment of PCR Method for Rapid Detection of Pathogenic Aeromonas hydrophila[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(12): 40-44.

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Establishment of PCR Method for Rapid Detection of Pathogenic Aeromonas hydrophila

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