贝类折光马尔太虫PCR检测方法的建立

Abstract

Abstract: According to the gene sequences in GenBank of Marteilia refringens, one pair of specific primers were designed for amplifying the specific fragments of Marteilia refringens. The reaction parameters were optimized to develop the polymerase chain reaction method for detection of Marteilia refringens. The 478 bp long specific DNA fragment of Marteilia refringens was amplified, but not from other pathogenic such as Perkinsus sp., Haplosporidium sp.,Aeromonas hydrophila, Pseudomonas fluorescens, Vibrio parahaemolyticu, Vibrio alginolyticu, and Vibrio fluvialis by this PCR. The sensitivity results showed that as little as 1 pg DNA of Marteilia refringens was detected by this PCR. 119 Oyster samples of Guangxi coastal were detected by this PCR. As a result, the positive rate was 1.68%. It suggested that Marteilia refringens existed in the cultivated shellfish in south China and the PCR method could be used as a sensitive tool to detect Marteilia refringens in clinical samples.

Key words: shellfish; Marteilia refringens; PCR

CLC Number:

S944

XIE Li-ji;XIE Zhi-xun;PANG Yao-shan;LIU Jia-bo;DENG Xian-wen;XIE Zhi-qin. Development of a Polymerase Chain Reaction Assay for Detection of Marteilia refringens in Shellfish[J]. , 2012, 39(1): 225-227.

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Development of a Polymerase Chain Reaction Assay for Detection of Marteilia refringens in Shellfish

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