Abstract: In order to promote the application and extension of artificial insemination technique in ostrich industry, secretion law of hormone was researched, which could be helpful to determine the most correct time of semen collection and insemination. In experiment, we used radioimmunoassay (RIA) to determine the concentration of follotropin (FSH), estrogen (E2) and testosterone (T) in sample. The results showed: ① During a year, the concentration of FSH in female ostrich plasma presented undulate changed. Concentration of FSH began to increase from November, and appeared three obvious wave crest of secretion at December, February and July, respectively 114.354, 130.168 and 144.375 mIU/mL. In middle of June, the level of FSH reached the lowest of the year, 22.312 mIU/mL;② The level of E2 ascended slowly from January to May, and drop to the lowest of the year in July suddenly(18.874 pg/mL), reached to the highest level of the year in September (367.285 pg/mL). ③ The concentration of T in male ostrich plasma ascended from January to June, reached a relatively high level in June (121.473 ng/dL) and reached the lowest level of the year in August (77.166 pg/dL),reached the highest level of the year in the middle of October (138.562 ng/dL). The concentration of FSH, E2 and T in plasma of ostrich presented undulate change during a year, which trended to be uniform with oestrus behavior.

Key words: ostrich; hormone; follotropin (FSH); estrogen (E2); testosterone