›› 2011, Vol. 38 ›› Issue (12): 100-102.

• 生物技术 • Previous Articles     Next Articles

Establishment of PCR Method for Quick Detection of Riemerella anatipestifer

WANG Xuan, XU Jing-e, PAN Shu-hui, WU Wei-heng, YANG Li   

  1. Institute of Animal Husbandry and Veterinary Medicine in Guizhou Province,Guiyang 550005, China
  • Received:1900-01-01 Revised:2011-06-02 Online:2011-12-20 Published:2011-12-20

Abstract: According to the gene sequences of the outer membrane protein A from Riemerella anatipestifer(RA) in GenBank, one pair of primers were selected and a specific PCR assay was made. Riemerella anatipestifer were found positive in the PCR. Staphyloccocus aureus, Rosenbach, Streptococcus, Pasteurella Multocida, Salmonella and Escherichia coli were all found negative in the PCR. The specificity and reproduction of PCR suggest it can be used for routine identification of Riemerella anatipestifer.

Key words: Riemerella anatipestifer; detection; PCR

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