快速检测双芽巴贝斯虫LAMP方法的建立

›› 2011, Vol. 38 ›› Issue (11): 113-116.

• 生物技术 • Previous Articles Next Articles

Rapid Detection of Babesia bigemina by Loop-mediated Isothermal Amplification

WANG Su-hua¹, CAI Wei-ming², LI Qun³, DU Ai-fang³

1. Wenzhou Entry-Exit inspection and Quarantine Bureau,Wenzhou 325027,China;2. Shaoxing Entry-Exit Inspection and Quarantine Bureau,Shaoxing 312000,China;3. Institute of Preventive Veterinary Medicine,Zhejiang University,Hangzhou 310029,China

Received:2011-04-06 Revised:1900-01-01 Online:2011-11-20 Published:2011-11-20

Abstract

Abstract: A loop-mediated isothermal amplification(LAMP) assay for rapid detection of Babesia bigemina was developed using primers specific to six distinct regions of Cytochrome b gene of Babesia bigemina. LAMP was performed using Bst DNA polymerase at 65 ℃ in water bath for 60 min and amplification results was visualized with SYBR Green Ⅰ. Specific bands could be detected for Babesia bigemina,but not for others. The detection limit of the assay was 0.085 fg/μL for Babesia bigemina,which was up to 1000 times higher than that of PCR methods. The LAMP methods was rapid and could be easily performed at a low cost.

Key words: Babesia bigemina; loop-mediated isothermal amplification (LAMP); detection; cyt b gene

CLC Number:

S855.9

WANG Su-hua;CAI Wei-ming;LI Qun;DU Ai-fang. Rapid Detection of Babesia bigemina by Loop-mediated Isothermal Amplification[J]. , 2011, 38(11): 113-116.

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Rapid Detection of Babesia bigemina by Loop-mediated Isothermal Amplification

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