Abstract: The specific primers were designed according to the published sequence of the infectious bovine rhinotracheitis virus (IBRV)gG gene. The method could amplify a specific IBRV fragment of 463 bp for IBRV reference strain and the IBRV positive samples. For bovine herpesvirus 5,it amplified two bands of 651 bp and 431 bp;for pseudorabies,the PCR product is a fragment of 493 bp. However,this PCR did not yield any product for non-related viruses such as porcine reproductive and respiratory syndrome virus. The sensitivity for IBRV was 2×10^-3 PFU/mL. Since this PCR has good sensitivity and specificity,it would have a wide application in diagnosis of bovine herpesvirus infection and differential detection among the members of herpesviruses and between natural infection and vaccination of gG deleted marker vaccine. A PCR method was established successfully to detect infectious bovine rhinotracheitis virus (IBRV) and differentiate bovine herpesviruses.

Key words: infectious bovine rhinotracheitis virus (IBRV); PCR; bovine herpesvirus; gG gene