›› 2010, Vol. 37 ›› Issue (7): 149-152.

• 疾病防治 • Previous Articles     Next Articles

Establishment of Nested-PCR Assay for Detection of Duck Circovirus

CAI Rui1，2，ZHANG Xiao-fei2，PAN Ling1，HU Lai-gen2，MAO Huo-yun2，YIN Xiu-feng2，HUANG Xian-ming2   

  1. (1.College of Animal Science and Technology, Anhui Agricultural University,Hefei 230036,China; 2.Nanjing Tianbang Bio-industry Co. Ltd, Nanjing 211102,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20
  • Contact: ZHANG Xiao-fei

Abstract: According to the sequence of duck circovirus (DuCV) in GenBank (AY228555),two pairs of primers were designed by Primer Premier 5.0.A nested-PCR assay for rapid detection of DuCV was established.The results showed that a specific 340 bp fragment was amplified from liver,thymus gland,bursa of Fabricius,kidney,spleen collected from Wangjiang county of Anhui province,but no bands were amplified with templates extracted respectively from normal duck embryo,healthy duck liver tissue,duck plague virus (DPV),avian influenza virus (AIV subtype H9),Newcastle disease virus (NDV),infectious bursal disease virus (IBDV),reticuloendotheliosis virus (REV),chicken infectious anemia virus (CAV),Escherichia coli (E.coli) of duck origin and Riemerella anatipestifer(RA).Sensitivity of the first and second amplifications by the nested-PCR assay was 1 ng and 1 fg,respectively.The sensitivity of the second amplifications was increased by 106 times.Results showed that the nested-PCR assay could be used as a method for the diagnosis and detection of clinical cases of DuCV and epidemiological investigation.

Key words: duck circovirus; nested-PCR; detection

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