利用CTC染色进行猪精子不同体外获能方法的比较

China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (4): 171-173.

• 遗传繁育 • Previous Articles Next Articles

Comparison of Different Methods for Boar Sperm Capacitation by CTC Staining

LIANG Hong-bin¹，LIU Jing-qing²，LI Fu-huang³， XU Hai-tao⁴，NIU Jia-ying¹， LI Jing¹， SUN Yang¹，GUO Yong¹，LIU Yun-hai¹

(1.Department of Animal Science & Technology，Beijing University of Agriculture,Beijing 102206，China；2. Yancheng AnimalHusbandry and Veterinary Medicine Administration Bureau of Jiangsu Province,Yancheng 224002，China；3.Beijing General Station of Animal Husbandry and Veterinary Medicine,Beijing 100107，China；4. Center of Preservation and Utilization of Germplasm Resources of Animal Husbandry and Forage Grass,National Husbandry and Veterinary Service,Beijing 100193,China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
Contact: LIU Yun-h

Abstract

Abstract: his study was aimed to explore the effects of different methods for boar sperm capacitation by chlortetracycline fluorescence (CTC) staining.Depending on the fresh boar sperm,the discrepancy of their capacitation were compared among the induction by swim-up,A23187 and caffeine incubation for different durations by CTC staining.The results showed that the rate of sperm capacitation by swim-up method and incubating for 40 min,were significantly higher than the others (P＜0.05).It could be concluded that the swim-up method was exactly suitable for the application of sperm capacitation for in vitro fertilization of swine.

Key words: boar; semen; sperm capacitation; chlortetracycline fluorescence (CTC) staining

CLC Number:

S814.4

LIANG Hong-bin;LIU Jing-qing;LI Fu-huang;XU Hai-tao;NIU Jia-ying;LI Jing;SUN Yang;GUO Yong;LIU Yun-hai. Comparison of Different Methods for Boar Sperm Capacitation by CTC Staining[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(4): 171-173.

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Comparison of Different Methods for Boar Sperm Capacitation by CTC Staining

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