›› 2010, Vol. 37 ›› Issue (2): 87-89.

• 生物技术 • Previous Articles     Next Articles

Principles and Application of Real- time Fluorescent Quantitative PCR Technique

ZHOU Xiao- li1,2, ZHU Guo- po2, LI Xue- hua2, WANG Yan- ling1, LIU Xing- you   

  1. (1.Henan Agricultural University, Zhengzhou 450002, China; 2.Henan Institute of Science and Technology, Xinxiang 453003, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

Abstract: Abstract: Real- time quantitative PCR is a qualitative PCR technique developed on the basis of the nucleic acid quantification. The qualities to be more specific, less polluted, and highly automated. This paper reviews main principles, and detection and diagnosis in pathogens and tumors of real- time fluorescence quantitative PCR.

Key words: Key words: real- time quantitative PCR; principles; detection; application