副猪嗜血杆菌环介导等温扩增检测方法的建立

›› 2010, Vol. 37 ›› Issue (2): 204-206.

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Establishment of Loop-mediated Isothermal Amplification for Detection of Haemophilus parasuis

ZHU Jie-yi1, TAN Shi-yong1, ZENG Xiao-na1, LIU Jian1, LIU Zhong-yong2, ZHU Dao-zhong2, LUO Man-lin1

（1.Guangdong Key Laboratory of Zoonosis, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; 2.Guangdong Entry-exit Inspection and Quarantine Bureau, Guangzhou 510623, China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

Abstract

Abstract: Abstract：Based on a series of 16S rRNA sequences of Haemophilus parasuis (Hps) published in GenBank, a conservative region was found. Four specific primers targeting the 6 distinct sequences of the conservative region were designed. A loop-mediated isothermal amplification (LAMP) for the rapid detection of Hps was established by using those specific primers. The results show that LAMP has a high specificity, and is more sensitive than PCR method under 63 ℃. Compared with the complex isolation and identification methods of Hps, LAMP is simple, low cost, and could be completed in 1 h. 

Key words: Key words：Haemophilus parasuis; 16S rRNA; loop-mediated isothermal amplification

ZHU Jie-yi; TAN Shi-yong; ZENG Xiao-na; LIU Jian; LIU Zhong-yong; ZHU Dao-zhong; LUO Man-lin. Establishment of Loop-mediated Isothermal Amplification for Detection of Haemophilus parasuis[J]. , 2010, 37(2): 204-206.

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Establishment of Loop-mediated Isothermal Amplification for Detection of Haemophilus parasuis

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