贝类单孢子虫PCR检测方法的建立

Abstract

Abstract: According to the gene sequences in GenBank of Haplosporidium sp, a pair of specific primers were designed for amplifying the specific fragments of Haplosporidium sp. The reaction parameters were optimized to develop the polymerase chain reaction method for detection of Haplosporidium sp. The 244 bp long specific DNA fragment of Haplosporidium sp was amplified, but not from other pathogenic such as Perkinsus sp, Marteilia refringens, Aeromonas hydrophila, Pseudomonas fluorescens, Vibrio parahaemolyticu, Vibrio Alginolyticu, Vibrio Fluvialis and Vibrio Mimicus by PCR. The sensitivity results showed that as little as 100 fg DNA of Haplosporidium sp was detected by this PCR.

Key words: Shellfish; Haplosporidium sp; PCR

CLC Number:

S852.7

XIE Li-ji;XIE Zhi-xun;PANG Yao-shan;LIU Jia-bo;DENG Xian-wen;XIE Zhi-qin. Development of A Polymerase Chain Reaction Assay for Detection of Haplosporidium in Shellfish[J]. , 2010, 37(1): 54-56.

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Development of A Polymerase Chain Reaction Assay for Detection of Haplosporidium in Shellfish

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