Abstract: A recombinant Doppel plasmid without glycosyl phosphatidyl inositol (pDoppel 1-155) was digested by Not Ⅰ,then the objective gene was recovered and purified. The gene was introduced into male pronucleus of BALB/c mouse fertilized ovum by microinjection, then we got 32 mice which have been injected the Dpl 1-155 gene, in which 6 was positive by PCR; one F0 generation of Tg mouse expressing Dpl without GPI was mated with a wild-type mouse, then 21 F1 generation mouse were got, in which 10 were positive by PCR identification. Brain tissue of three F1 generation mice which were positive by PCR identification was collected, and was identified by Western blotting, there was 17 ku Dpl 1-155 protein expressed in the brain. So we have generated Tg mice expressing Dpl without GPI which provides Tg animal models for investigating the bionomics of Dpl protein.

Key words: glycosyl phosphatidyl inositol; Doppel; transgene; mouse