Wip1过表达转基因小鼠模型的制备与鉴定

doi:10.16431/j.cnki.1671-7236.2016.12.002

Abstract

Abstract:

This study was aimed to build Wip1 over-expressed transgene mice, which could provide an animal model for clarifying the pathogenetic mechanism of atherosclerosis, and the transgene mice were screened at RNA level. Mice tissues cDNA were obtained by reverse transcription polymerase chain reaction (RT-PCR), which were used for the next PCR amplification template. The amplified fragments were used for gel recovery.Then, the amplified fragment and empty vector were double enzyme digested.Full-length gene of Wip1,which was obtained by gel recovery, was connected to pcDNA3.1(+) vector, and the Wip1-pcDNA3.1(+) over-expressed vector was constructed. Finally, Wip1 over-expressed transgene mice were obtained. The transgene mice were identified and screened by Southern blotting and qPCR. 30 Wip1 over-expressed transgene mice were got in this experiment, and the positive mice identified were 11 by Southern blotting, the positive rate was 36.67%. Moreover,the positive rate was 32.84% through screening by qPCR. Therefore, the Wip1-pcDNA3.1(+) over-expressed vector was constructed successfully, and the Wip1 over-expressed transgene mice were obtained through identifying and screening at DNA and RNA level of Wip1 in mice tissues.

Key words: Wip1; over-expression; mouse model

CLC Number:

GAO Qian, HU Yan-qing, TANG Yi-ting, MU Yu-lian. Establishment and Identification of Wip1 Over-expressed Transgene Mice Model[J]. , 2016, 43(12): 3094-3100.

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Establishment and Identification of Wip1 Over-expressed Transgene Mice Model

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