一株鹅Ⅰ型副粘病毒全基因克隆与序列分析

›› 2009, Vol. 36 ›› Issue (4): 80-85.

• 生物技术 • Previous Articles Next Articles

Cloning and Genome-sequence Analysis of a Goose Paramyxovirus Type

SONG Cui-ping1, ZHOU Jian1,2, WANG Xiao-jiao1, CUI Shang-jin3

（1.China Animal Health and Epidemiology Center, Qingdao 266032, China; 2.Animal Husbandry Bureau of Anqiu Town in Shandong Province, Anqiu 262103, China; 3.National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China）

Received:2008-09-25 Revised:1900-01-01 Online:2009-04-20 Published:2009-04-20
Contact: CUI Shang-jin

Abstract

Abstract: Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the six major encoding structure protein of an A goose paramyxovirus type I (ZQ042). The cDNAs were then cloned to pMD18-T vector and sequenced. These sequences were compared with paramyxovirus type I home and abroad reported. The results showed that the F gene was comparatively far related with La Sota, rate of homologous was 99.1%, and other genetic homology of the strains was low.

Key words: paramyxovirus; gene; cloning; sequencing

CLC Number:

Q785

SONG Cui-ping;ZHOU Jian;;WANG Xiao-jiao;CUI Shang-jin. Cloning and Genome-sequence Analysis of a Goose Paramyxovirus Type[J]. , 2009, 36(4): 80-85.

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Cloning and Genome-sequence Analysis of a Goose Paramyxovirus Type

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