China Animal Husbandry and Veterinary Medicine ›› 2024, Vol. 51 ›› Issue (2): 443-452.doi: 10.16431/j.cnki.1671-7236.2024.02.001

• Biotechnology • Previous Articles     Next Articles

Expression and Bioinformatics Analysis of Cat CDH1 Gene in c-MYC Overexpressed Fibroblasts

AN Jie1, YANG Jie1, DOU Minmin2, SUN Nannan1, ZHAO Dipeng2, DU Rong2, QIN Jian1,2,3   

  1. 1. College of Life Science, Shanxi Agricultural University, Taigu 030801, China;
    2. College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
    3. Center of Experiment Teaching, Shanxi Agricultural University, Taigu 030801, China
  • Received:2023-08-03 Online:2024-02-05 Published:2024-01-29
  • Contact: 山西省基础研究计划(自由探索类)自然科学研究面上项目(20210302123394);山西省回国留学人员科研资助项目(2022-102);国家自然科学基金项目(32272964、31872438);山西省留学回国人员科技活动择优资助项目;山西农业大学中青年拔尖创新人才支持计划(BJRC201204);山西农业大学横向科技项目(2015HX12)
  • Supported by:
    The project was supported by the National Key Research and Development Program of China (2019YFC1905301);National Natural Science Foundation of China (22078115,21776108,21690083,22008078).

Abstract: 【Objective】 This study was aimed to investigate the effect of myelocytomatosis viral oncogene homolog (c-MYC) on cat fibroblasts and its relationship with cadherin 1 (CDH1) gene expression and molecular characteristics, so as to provide a basis for application in the prevention and treatment of tumor diseases and the repair of tissue damage in cat.【Method】 Cat fetal fibroblasts were isolated and cultured by tissue adherence, and the PB-TRE-c-MYC plasmid was transfected into the cells by electroporator, then the cell morphology was observed.The expression of CDH1 gene was detected by Real-time quantitative PCR, and the physicochemical properties and structural characteristics of CDH1 protein were analyzed by bioinformatics software.【Result】 c-MYC overexpression led to the changes in cell morphology, with a transition from the long shuttle shape of mesenchymal cells to the pebble shape of epithelial cells, and the expression of epithelial cell marker gene CDH1 was extremely significantly upregulated (P<0.01).Bioinformatics analysis results showed that CDH1 protein in cat had 881 amino acids, of which the most abundant was leucine, localized in cell membrane, with 4 CA domains, which mediated cell-cell contact.CDH1 protein belonged to hydrophilic acidic proteins, mainly composed of random coil, and might have signaling peptide sites transported by Sec translocons and cleaved by signal peptidase Ⅰ (Sec/SPⅠ).【Conclusion】 The expression of CDH1 gene in cat could be activated by exogenous reprogramming factor c-MYC, and its encoding protein cadherin could promote the mesenchymal-to-epithelial transition of cat fetal fibroblasts to inhibit cell carcinogenesis.

Key words: cat; c-MYC gene; CDH1 gene; mesenchymal-to-epithelial transition

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