广东地区畜禽源大肠杆菌的耐药性与流行性研究

doi:10.16431/j.cnki.1671-7236.2024.02.040

Abstract

Abstract: 【Objective】 The aim of this experiment was to investigate the prevalence and drug resistance of Escherichia coli (E. coli) from livestock and poultry in some areas of Guangdong, and provide reference for drug resistance prevention and control of E. coli derived from animals.【Method】 Samples of feed, drinking water and anal (cloaca) swabs of healthy animals collected from 10 farms in some areas of Guangdong in 2019 were subjected to E. coli isolation and identification, drug sensitivity test, detection of drug resistance genes and insertion sequence common region (ISCR), as well as ethnic phylogenetic analysis.【Result】 A total of 416 E. coli strains were isolated, including 78 strains from breeding pigs, 77 strains from broilers, 122 strains from laying hens and 139 strains from broilers.These strains showed the characteristics of multiple drug resistance, and the drug resistance rate of E. coli from broilers was the highest among different animal sources.79.6% (331/416) strains were resistant to 3 or more drugs, up to 13 drugs, and a total of 156 drug resistance profiles were formed.The main resistance profiles was sulfamethoxazole-trimethoprim/florfenicol/doxycycline/amoxicillin/ampicillin, accounting for 11.1%. floR gene showed the highest detection rate of 50.0%, followed by sul2 and sul3 genes, which were 44.2% and 42.5%, respectively.The detection rates of tet(X3), tet(X4) and mcr-1 genes associated with tigecycline and colistin resistance phenotypes were all lower than 10%.Insert sequence common region (ISCR) elements for ISCR1, ISCR2 and ISCR3 were present in 14.4%, 10.8% and 10.6% isolates, respectively.The coincidence rate of phenicols, sulfonamides and β-lactam resistance genes with drug-resistant strains was more than 60%, respectively.The coexistence of drug resistance genes cmlA, tetA, qnrS, bla_CTX-M-9G with ISCR1, tet(X4), sul2 with ISCR2, and tet(X4), sul3, qnrS, bla_SHV with ISCR3, were statistically significant (P＜0.05), respectively.E. coli from different animals and ISCR-related mobile element-positive bacteria were both mainly distributed in phylogenetic group A.【Conclusion】 E. coli isolates from animals in some areas of Guangdong presented multiple drug resistance, and carried multiple drug resistance genes, as well as mobile elements ISCR.These results provided reference for understanding the drug resistance characteristics and prevalence of resistant genes in animal-derived bacteria in Guangdong province.

Key words: Escherichia coli; livestock and poultry; drug resistance; resistance genes

CLC Number:

S852.61⁺2

LI Yafei, LIANG Xiaoyun, WU Liqin, XIAO Tianan, WANG Fuhua, ZENG Zhenling. Drug Resistance and Prevalence of Escherichia coli Isolated from Livestock and Poultry in Areas of Guangdong[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 837-849.

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Drug Resistance and Prevalence of Escherichia coli Isolated from Livestock and Poultry in Areas of Guangdong

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