鸭源H5N1亚型高致病性禽流感病毒的分离鉴定及其HA和NA基因的生物信息分析

›› 2009, Vol. 36 ›› Issue (2): 62-67.

• 生物技术 • Previous Articles Next Articles

Identification and Biological Information Analysis of H5N1 Subtype High  Pathogenic Avian Influenza Virus Isolated from Duck

SUN En-cheng1, LIU Chun-guo2，HOU Xi-lin1，LIU Ming2，PIAO Fan-ze1

（1.Heilongjiang August First Land Reclamation University, Academy of Animal Science and Technology, Daqing 163319, China； 2.Animal Influenza Laboratory of the Ministry of Agriculture, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences， Harbin 150001, China）

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-20 Published:2009-02-20
Contact: HOU Xi-lin

Abstract

Abstract:

A H5N1 subtype avian influenza virus isolated from duck belongs to high pathogenic avian influenza virus. HA and NA gene of the solated strain shares 98.8% and 98.7% nucleotide sequence identity to the HA gene of A/duck/VietNam/Ncvd1/2002(H5N1) and the NA gene of A/duck/VietNam/Ncvd1/2002(H5N1) and A/chicken/Jiangsu/cz1/2002(H5N1), and shares 99. 3% and 98.7% amino acid sequence identity to the HA protein of A/duck/Viet Nam/Ncvd1/2002(H5N1) and the NA protein of A/chicken/Jiangsu/cz1/2002(H5N1). There is several continuous basic amino acid(PQREIRRKKR*G) at the cleavage site of HA protein show its a high pathogenic avian influenza virus, and compared with the other influenza viruses the isolated strain has no difference in Nglycosylation sites. Phylogenetic trees analysis of HA and NA gene suggests that the isolated influenza virus probably originated from the A/duck/Viet Nam/Ncvd1/2002(H5N1).

Key words: avian influenza virus; HA gene; NA gene; biological information analysis

CLC Number:

S855.3

SUN En-cheng;LIU Chun-guo;HOU Xi-lin;LIU Ming;PIAO Fan-ze . Identification and Biological Information Analysis of H5N1 Subtype High  Pathogenic Avian Influenza Virus Isolated from Duck[J]. , 2009, 36(2): 62-67.

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Identification and Biological Information Analysis of H5N1 Subtype High  Pathogenic Avian Influenza Virus Isolated from Duck

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