China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (12): 57-59.

• 生物技术 • Previous Articles     Next Articles

PCR Amplification and Sequence Analysis of Mitochondrial Cytochrome c Oxidase Subunit 1 (cox1) of Syphacia obvelata from Mice

LI Xin, CHEN Xiao-mei, FAN Wen-ying, DENG Jun-you,SONG Hui-qun, ZHU Xing-quan, LIN Rui-qing   

  1. (College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-12-20 Published:2009-12-20
  • Contact: LIN Rui-qing

Abstract: The partial sequences of mitochondrion cox1 (pcox1) gene of 5 pinworm samples separated from mice were amplified by PCR and the positive products were sequenced. Then sequences were analysed by BLAST and compared with Syphacia obvelata and S. montana available in GenBank. The results revealed that the length of the pcox1 sequence was 373 bp. Sequence alignments showed that genetic variation within 5 pinworm samples was very small, with only one mutation locus, and these samples were identified as S. obvelata by BLAST. The inter-specific sequence difference was 5.24%-5.28% between S. obvelata samples and S. montana available in GenBank. These findings showed that the intra-specific variations of cox1 were low for S. obvelata and the inter-specific sequence differences were comparatively higher between S. obvelata and S. montana. The results provided foundation for further studies of population genetics of pinworms. 

Key words: pinworm; mitochondrial DNA; cytochrome c oxidase subunit 1（cox1）gene; PCR

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