猪繁殖与呼吸综合征病毒M基因RT-LAMP检测方法的建立

›› 2009, Vol. 36 ›› Issue (12): 53-56.

• 生物技术 • Previous Articles Next Articles

Establishment of Reverse Transcriptase Loop-mediated Isothermal Amplification for Detection of Porcine Reproductive and Respiratory Syndrome Virus M Gene

ZHAO Yan-zong, ZHANG Xian-hao, YU Xiao-li, SU Dan-ping, HE Dong-sheng

（Guangdong Key Laboratory of Zoonosis, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China）

Received:1900-01-01 Revised:1900-01-01 Online:2009-12-20 Published:2009-12-20
Contact: HE Dong-sheng

Abstract

Abstract: A series of specific primers targeting the 6 distinct sequences of M gene were designed based on the sequences of porcine reproductive and respiratory syndrome virus (PRRSV) GP6 (M) gene available in GenBank. A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) for the rapid detection of PRRSV was developed by using the specific primers. The results showed that the detective sensitivity of RT-LAMP method was higher than that of the two-step RT-PCR. The amplification could be accomplished within 1 h with good specificity and high sensitivity, while no product was generated with porcine circovirus, classic swine fever virus and pseudorabies virus. With the addition of SYBR GREENⅠ, the PT-LAMP of PRRSV could be detected by naked eyes.

Key words: porcine reproductive and respiratory syndrome virus; M gene; reverse transcriptase loop-mediated isothermal amplification (RT-LAMP)

CLC Number:

S852.65

ZHAO Yan-zong;ZHANG Xian-hao;YU Xiao-li;SU Dan-ping;HE Dong-sheng. Establishment of Reverse Transcriptase Loop-mediated Isothermal Amplification for Detection of Porcine Reproductive and Respiratory Syndrome Virus M Gene[J]. , 2009, 36(12): 53-56.

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Establishment of Reverse Transcriptase Loop-mediated Isothermal Amplification for Detection of Porcine Reproductive and Respiratory Syndrome Virus M Gene

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