栀子四黄散抗猪繁殖与呼吸综合征的研究

doi:10.16431/j.cnki.1671-7236.2020.11.026

Abstract

Abstract: In order to investigate the therapeutic effect of Gardenia Sihuang powder on porcine reproductive and respiratory syndrome,45 pigs were randomly divided into five groups:Control group,virus group,low dose Chinese medicine prescription group,the middle dose Chinese medicine prescription group and the high dose Chinese medicine prescription group.Experimental pigs with porcine reproductive and respiratory syndrome virus (PRRSV) were added to each herb after 400 MTS of ultricro grinding,After continuous feeding for 14 days,clinical symptoms were observed daily.Five groups of serum samples were randomly collected for cytokine detection at 7,14 and 21 d,and pathological autopsy and lung samples were collected for pathological tissue section observation.The results showed that after adding traditional Chinese medicine for 7 days,the clinical symptoms were all relieved.At 14 days,the body temperature returned to normal,the symptoms of dyspnea disappeared,and the appetite recovered in middle and high dose groups.No meat-like changes were observed in the lungs of the traditional Chinese medicine treatment group,and there were bleeding spots or blood spots on the surface of the lungs.In the middle dose group,alveolar septa were thickened,blood congestion was mild,and lung parenchyma was mildly inflammatory cell infiltration.The levels of IgG and IFN-γ in the serum of the virus group decreased gradually,while the levels of IL-10 increased gradually.The contents of IgG in the serum of the three groups added with traditional Chinese medicine were higher than that of the virus group,and the content of IgG gradually increased.The content of IgG in the serum of the middle dose group was extremely significantly higher than that of the control group and the virus group (P<0.01).The content of IFN-γ in the three groups with traditional Chinese medicine gradually increased,and the serum content of IFN-γ in the middle dose group was extremely significantly higher than that of the control group and the virus group (P<0.01).The serum IL-10 content in the middle dose group gradually decreased,and the difference with the virus group was extremely significant (P<0.01),but not significant with the control group (P>0.05) at 14 and 21 days.The results of this study showed that fructus Gardenia Sihuang powder could effectively improve clinical symptoms,pathological changes and cytokine secretion in experimental pigs.It provided experimental basis for the prevention and treatment of porcine reproductive and respiratory syndrome.

Key words: porcine reproductive and respiratory syndrome virus (PRSSV); IgG; IFN-γ; IL-10; fructus Gardenia Sihuang powder

CLC Number:

S854.5⁺1

XIE Huimei, CAI Bingyan, HAO Fuxing, GUO Guangfu, JIANG Wenqing, LIU Jianing, CHENG Han. Study on Resistance of Fructus Gardeniae Sihuang Powder to Porcine Reproductive and Respiratory Syndrome[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3633-3640.

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Study on Resistance of Fructus Gardeniae Sihuang Powder to Porcine Reproductive and Respiratory Syndrome

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