金堂黑山羊白介素10基因的克隆及表达分析

doi:10.16431/j.cnki.1671-7236.2018.12.008

Abstract

Abstract:

This study was aimed to explore the sequence characteristics of interleukin 10 (IL-10) gene and its relative expression of different tissues in goat.IL-10 gene of Jintang Black goat was amplified and cloned by PCR,the gene sequence was analyzed by bioinformatics softwares such as DNAMAN,DNAStar and ExPASy,then the expression of IL-10 gene in different tissues was detected by Real-time quantitative PCR.The results showed that the sequence of IL-10 gene was 379 bp,and the open reading frame was 276 bp,encoding 91 amino acid residues.The secondary structure of IL-10 protein contained alpha helix(76.92%),beta turn(2.20%) and random coil(20.88%).The tertiary structure model of IL-10 protein had the highest homology with human IL-10 (1ilk.1.A) gene (86.81%).In the phylogenetic tree,IL-10 gene in Jintang Black goat was closely related to Norwegian goat.Real-time quantitative PCR result showed that the expression level of IL-10 gene in lung of Jintang Black goat was significantly higher than that in spleen,heart,muscle and kidney (P<0.05),the expression of IL-10 gene in kidney was significantly higher than that in spleen,heart and muscle (P<0.05),the expressions of IL-10 gene in spleen,heart and muscle were lower,but there was no significant difference (P>0.05).It revealed that IL-10 gene was highly conserved,and it might be involved in regulating the immune response of goats.

Key words: Jintang Black goat; IL-10 gene; cloning; expression analysis

CLC Number:

FANG Qing, WANG Li. Cloning and Expression Analysis of Interleukin 10 Gene in Jintang Black Goat[J]. , 2018, 45(12): 3371-3378.

References

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Cloning and Expression Analysis of Interleukin 10 Gene in Jintang Black Goat

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