地衣芽孢杆菌对玉米青贮发酵品质的调控研究

doi:10.16431/j.cnki.1671-7236.2025.03.009

Abstract

Abstract: 【Objective】 Silage bacteria preparation can improve silage quality,feed utilization and animal digestibility,at the same time,it can effectively inhibit the activity of harmful microorganisms,thereby improving the palatability of silage.Bacillus licheniformis plays a key role in the early aerobic fermentation stage of corn silage with its unique biological oxygen capture,which can significantly regulate the silage quality and ensure the stability of the whole fermentation process and the quality of the final product.The purpose of this study was to investigate the effects of different concentrations of Bacillus licheniformis on the quality and mycotoxin of corn silage,in order to provide reference for the application of Bacillus licheniformis in silage.【Method】 Whole silage maize was used as raw material in the experiment.A completely randomized block design was adopted,and control group (without adding Bacillus licheniformis,CK),group Ⅰ (adding 0.8×10⁶ CFU/g DM Bacillus licheniformis),group Ⅱ (adding 1.6×10⁶ CFU/g DM Bacillus licheniformis) and group Ⅲ (adding 3.2×10⁶ CFU/g DM Bacillus licheniformis) were set up,each group had 3 replicates.After silage for 60 days,the nutritional composition,fermentation quality,feeding value,mycotoxin and comprehensive quality of corn silage in each group were analyzed.【Result】 ① Compared with CK group,Bacillus licheniformis had no significant effect on the content of dry matter,crude protein,starch,soluble carbohydrates and ash in corn silage (P＞0.05),the fat content of corn silage in group Ⅲ was significantly increased (P＜0.05),and the contents of neutral detergent fiber and acid detergent fiber of corn silage in groups Ⅱ and Ⅲ were significantly decreased (P＜0.05).② There was no significant difference in lactic acid content among all treatment groups (P＞0.05).The NH₃-N content of corn silage in group Ⅲ was significantly lower than that in CK group (P＜0.05).Bacillus licheniformis significantly affected pH and acetic acid content of corn silage (P＜0.05),and with the increase of Bacillus licheniformis,pH showed a linear decrease,while the acetic acid content showed a linear and quadratic curve increase (P＜0.05).③ The comprehensive analysis by the fuzzy membership function method showed that corn silage in group Ⅲ had the highest membership function mean value.④ Compared with CK group,the contents of vomitoxin,fumonitoxin and aflatoxin in experimental groups were significantly decreased (P＜0.05),but there was no significant effect on the contents of zeralenone and T2-toxin (P＞0.05),and the contents of vomitoxin and fumonitoxin were linearly and conically decreased with the increase of Bacillus licheniformis supplementation (P＜0.05).【Conclusion】 Under the conditions of this experiment,Bacillus licheniformis could improve the quality of corn silage,especially when 3.2×10⁶ CFU/g Bacillus licheniformis was added.

Key words: Bacillus licheniformis; corn silage; fermentation quality; mycotoxin; membership function

CLC Number:

S816.5⁺3

WANG Rui, HEJintong, CHENG Yuchen, REN Wenyi, MA Yulin, XU Xiaofeng. Study on Regulating the Fermentaiton Quality of Corn Silage by Bacillus licheniformis[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1070-1079.

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Study on Regulating the Fermentaiton Quality of Corn Silage by Bacillus licheniformis

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