3种铝硅酸盐类吸附剂及甘露低聚糖对5种霉菌毒素的吸附效果评价

doi:10.16431/j.cnki.1671-7236.2025.01.017

Abstract

Abstract: 【Objective】 Feed in the storage process due to moisture content,environmental temperature,humidity and other factors,feed is prone to mildew and produce mycotoxins,how to reduce the mycotoxins contamination of feed is an urgent problem to be solved currently.The aim of this experiment was to study the effects of three aluminosilicate adsorbents,namely attapulgite,permutite and montmorillonite,and mannooligosaccharides on five toxins,namely,aflatoxin B₁ (AFB₁),deoxynivalenol (DON),zearalenone (ZEN),ochratoxins A (OTA) and T-2 toxin in vitro. 【Method】 The adsorption rates of attapulgite,permutite,montmorillonite and mannooligosaccharides at the addition of 0.25%,0.5%,1% and 2%,respectively,as well as the adsorption and desorption rate of each adsorbent at different pH were determined by enzyme-linked immunosorbent assay (ELISA). 【Result】 ① The addition amount,adsorbent and interaction between the two had extremely significant effect on the adsorption rates of five toxins (P<0.01).Among them,compared with the other three additions,the adsorption rate of five toxins was extremely significantly higher at 2% addition (P＜0.01),the adsorption rate of montmorillonite on five toxins was extremely significantly higher than that of attapulgite,permutite and mannooligosaccharides (P＜0.01).② Under the condition of the same additive amount,the adsorption rates of AFB₁ by mannooligosaccharide,attapulgite and montmorillonite at pH 10.0 were extremely significantly higher than those at pH 4.0 (P＜0.01),the adsorption rate of four adsorbents on DON were extremely significantly higher at pH 10.0 than those at pH 4.0 (P＜0.01).Permutite adsorption of OTA at pH 10.0 was extremely significant higher than that at pH 4.0 (P＜0.01),and the OTA adsorption rate of attapulgite at pH 4.0 was extremely significantly higher than that of pH 10.0 (P＜0.01).At 1% addition,four adsorbents adsorbed ZEN and T-2 toxins at pH 10.0 were extremely significantly higher than those at pH 4.0 (P＜0.01).At the addition of 0.5%,mannooligosaccharides and permutite adsorbed ZEN at pH 10.0 were extremely significantly higher than those at pH 4.0 (P＜0.01), and attapulgite,permutite and montmorillonite adsorbed T-2 toxin at pH 4.0 were extremely significantly higher than those at pH 10.0 (P＜0.01).③ After adding PBS with pH 7.0 to the centrifugal precipitation at pH 4.0,the desorption rate of montmorillonite was extremely significantly lower than that of attapulgite and permutite (P＜0.01).After adding methanol to the centrifugal precipitation at pH 7.0 again,the desorption rates of montmorillonite and permutite were extremely significantly lower than those of attapulgite (P＜0.01). 【Conclusion】 The adsorption rates of four adsorbents on AFB₁,DON,ZEN,OTA and T-2 toxins at 2% addition were montmorillonite ＞ permutite ＞ attapulgite ＞ mannooligosaccharides in descending order.Four adsorbents adsorbed higher on AFB₁,DON and ZEN at pH 10.0 and 1% addition,and after the adsorption environment at pH 4.0 was changed to pH 7.0 or methanol,the adsorption rate of three adsorbents on DON,ZEN,OTA and T-2 toxins were montmorillonite ＞ permutite ＞ attapulgite in desorption rate from low to high.

Key words: adsorbent; mannooligosaccharides; mycotoxin; ELISA

CLC Number:

S816.79

YU Yingying, WANG Caidie, ZANG Changjiang, LI Mengfei, XU Zihao, SUN Long, HOU Min, LI Xiaobin, LI Fengming. Evaluation of Adsorption Effect of Three Aluminosilicate Adsorbents and Mannooligosaccharides on Five Mycotoxins[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 195-204.

References

[1] FRANCO L T,ISMAIL A,AMJAD A,et al.Occurrence of toxigenic fungi and mycotoxins in workplaces and human biomonitoring of mycotoxins in exposed workers:A systematic review[J].Toxin Reviews,2021,40(4):576-591.
[2] LIU L,XIE M,WEI D.Biological detoxification of mycotoxins:Current status and future advances[J].International Journal of Molecular Sciences,2022,23(3):1064.
[3] WU L,LIAO P,HE L,et al.Growth performance,serum biochemical profile,jejunal morphology,and the expression of nutrients transporter genes in deoxynivalenol (DON)-challenged growing pigs[J].BMC Veterinary Research,2015,11:144.
[4] ZAIN M E.Impact of mycotoxins on humans and animals[J].Journal of Saudi Chemical Society,2011,15(2):129-144.
[5] JI C,FAN Y,ZHAO L.Review on biological degradation of mycotoxins[J].Animal Nutrition,2016,2(3):127-133.
[6] 苏青云,齐莎日娜,雷元培,等.2022年中国饲料和原料中霉菌毒素污染调查报告[J].饲料工业,2024,45(9):121-125. SU Q Y,QI S R N,LEI Y P,et al.A survey report on the mycotoxin contamination of Chinese raw materials and feed in 2022[J].Feed Industry,2024,45(9):121-125.(in Chinese)
[7] 赵伟,厉学武,DESSALEGN L,等.我国饲料原料和配合饲料中T-2毒素、赭曲霉毒素A和伏马毒素的污染研究[J].动物营养学报,2024,36(4):2690-2698. ZHAO W,LI X W,DESSALEGN L,et al.A study of contamination of T-2 toxin,ochratoxin A and fumonisin in feed ingredients and compound feed in China[J].Chinese Journal of Animal Nutrition,2024,36(4):2690-2698.(in Chinese)
[8] 段海涛,卢丽枝,梁蒙蒙,等.不同吸附剂产品对黄曲霉毒素B₁及赤霉烯酮吸附效果评价[J].饲料工业,2023,44(15):100-105. DUAN H T,LU L Z,LIANG M M,et al.Evaluation of adsorption effect of different adsorbents on aflatoxin B₁ and earalenone[J].Feed Industry,2023,44(15):100-105.(in Chinese)
[9] ALSHANNAQ A,YU J.Occurrence,toxicity,and analysis of major mycotoxins in food[J].International Journal of Environmental Research and Public Health,2017,14(6):632.
[10] VILA-DONAT P,MARIN S,SANCHIS V,et al.A review of the mycotoxin adsorbing agents,with an emphasis on their multi-binding capacity,for animal feed decontamination[J].Food and Chemical Toxicology,2018,114:246-259.
[11] 张立阳,赵雪娇,刘帅,等.蒙脱石改性前后对3种常见霉菌毒素的吸附效果及结构表征[J].中国畜牧杂志,2019,55(3):86-90. ZHANG L Y,ZHAO X J,LIU S,et al.Adsorption capacity to three common mycotoxins and structural characterization of montmorillonite before and after modification[J].Chinese Journal of Animal Science,2019,55(3):86-90.(in Chinese)
[12] 施晶晶,李爱科,王薇薇,等.粮油及其加工副产物中霉菌毒素全产业链防控技术研究进展[J].中国粮油学报,2024.Doi:10.20048/j.cnki.issn.1003-0174.000766. SHI J J,LI A K,WANG W W,et al.Research progress on prevention and control technology of mycotoxins in grain,oil,and its processing by-products based on the whole industry chain[J].Journal of the Chinese Cereals and Oils Association,2024.Doi:10.20048/j.cnki.issn.1003-0174.000766.(in Chinese)
[13] 杨凡,张俊楠,王金全,等.霉菌毒素吸附剂对玉米赤霉烯酮脱毒效果的评价[J].动物营养学报,2020,32(5):2116-2125. YANG F,ZHANG J N,WANG J Q,et al.Assessment on detoxification effects of mycotoxin adsorbent on zearalenone[J]. Chinese Journal of Animal Nutrition,2020,32(5):2116-2125.(in Chinese)
[14] 齐德生,于炎湖.铝硅酸盐类吸附剂对霉菌毒素的选择性吸附机制及其应用[J].粮食与饲料工业,2002,5:31-33. QI D S,YU Y H.Efficiency and mechanism of aluminosilicate in reducing the toxicity of mycotoxins[J]. Cereal & Feed Industry,2002,5:31-33.(in Chinese)
[15] 罗自生,秦雨,徐艳群,等.黄曲霉毒素的生物合成、代谢和毒性研究进展[J].食品科学,2015,36(3):250-257. LUO Z S,QIN Y,XU Y Q,et al.Recent progress in the biosynthesis,metabolism and toxicity of aflatoxins[J].Food Science,2015,36(3):250-257.(in Chinese)
[16] PHILLIPS T D,SARR A B,GRANT P G.Selective chemisorption and detoxification of aflatoxins by phyllosilicate clay[J].Journal of Natural Toxins,1995,3(4):204-213.
[17] STANLEY V G,OJO R,WOLDESENBET S,et al.The use of Saccharomyces cerevisiae to suppress the effects of aflatoxicosis in broiler chicks[J].Journal of Poultry Science,1993,72(10):1867-1872.
[18] MAKI C R,MONTEIRO A P A,ELMORE S E,et al.Calcium montmorillonite clay in dairy feed reduces aflatoxin concentrations in milk without interfering with milk quality,composition or yield[J].Animal Feed Science and Technology,2016,214:130-135.
[19] MIAZZO R,PERALTA M F,MAGNOLI C,et al.Efficacy of sodium bentonite as a detoxifier of broiler feed contaminated with aflatoxin and fumonisin[J].Journal of Poultry Science,2005,84(1):1-8.
[20] 梁晓维,李发弟,张军民,等.蒙脱石和凹凸棒石对霉菌毒素吸附性能的研究[J].中国畜牧兽医,2014,41(11):133-138. LIANG X W,LI F D,ZHANG J M,et al.Study on adsorption of montmorillonite and attapulgite to mycotoxins[J].China Animal Husbandry & Veterinary Medicine,2014,41(11):133-138.(in Chinese)
[21] 唐胜球,董小英,郭燕珊,等.新型水产饲料添加剂——甘露低聚糖[J].广东饲料,2011,20(12):29-32. TANG S Q,DONG X Y,GUO Y S,et al.A new additive for aquatic feed — Mannooligosaccharide[J].Guangdong Feed,2011,20(12):29-32.(in Chinese)
[22] 徐瑞,王改琴,刘春雪,等.不同粘土矿物对霉菌毒素和营养物质的吸附和解吸效果研究[J].粮食与饲料工业,2021,6:43-46. XU R,WANG G Q,LIU C X,et al.Effects of different clay minerals on the adsorption and desorption of mycotoxins and nutrients[J]. Cereal & Feed Industry,2021,6:43-46.(in Chinese)
[23] WANG G,LIAN C,XI Y,et al.Evaluation of nonionic surfactant modified montmorillonite as mycotoxins adsorbent for aflatoxin B₁ and zearalenone[J].Journal of Colloid and Interface Science,2018,518:48-56.
[24] 张鹏,陈星月,李素芹,等.粉煤灰制备沸石的技术及应用现状[J].材料导报,2024,38(7):110-123. ZHANG P,CHEN X Y,LI S Q,et al.Development and research status of zeolite synthesized from coal fly ash[J].Materials Reports,2024,38(7):110-123.(in Chinese)
[25] 娄秀萍,黄晴雯,郭文博,等.氧化锌纳米颗粒光催化降解T-2毒素性能分析[J].食品科学,2024,45 (18):10-16. LOU X P,HUANG Q W,GUO W B,et al.Photocatalytic degradation T-2 toxin by zinc oxide nanoparticles catalyst[J].Food Science,2024,45(18):10-16.(in Chinese)
[26] DIAZ D E,HAGLER W M,HOPKINS B A,et al.Aflatoxin binders Ⅰ:In vitro binding assay for aflatoxin B₁ by several potential sequestering agents[J]. Mycopathologia,2003,156:223-226.
[27] 李娟娟,索德成,苏晓鸥.3种吸附剂对黄曲霉毒素B₁吸附能力的研究[J].中国农业科学,2009,42(11):4027-4034. LI J J,SUO D C,SU X O.Binding assay for aflatoxin B₁ by 3 absorbents[J].Scientia Agricultura Sinica,2009,42(11):4027-4034.(in Chinese)
[28] 杨慧莹.不同霉菌毒素吸附剂对黄曲霉毒素B₁的吸附效果研究[D].长春:吉林农业大学,2023. YANG H Y.Study on the adsorption effect of different mycotoxin adsorbents on aflatoxin B₁[D].Changchun:Jilin Agricultural University,2003.(in Chinese)
[29] 桑勇.几种有机吸附剂对黄曲霉毒素吸附的比较研究[D].武汉:华中农业大学,2006. SANG Y.Absorption of several organic sorbents for AFB₁ and their detoxification for AFB₁ in animal[D].Wuhan:Huazhong Agricultural University,2006.(in Chinese)
[30] 刘媛婷.霉菌毒素吸附剂对黄曲霉毒素B₁吸附特性及效果研究[D].雅安:四川农业大学,2011. LIU Y T.Study on adsorption characteristics and effects of mycotoxin adsorbents on aflatoxin B₁ in feed[D].Ya’an:Sichuan Agricultural University,2011.(in Chinese)
[31] 史莹华.纳米级硅酸盐结构微粒(NSP)吸附猪饲粮中黄曲霉毒素的研究[D].杭州:浙江大学,2005. SHI Y H.Study on adsorption of dietary aflatoxin by nanometer silicate particle (NSP) in swine[D].Hangzhou:Zhejiang University,2005.(in Chinese)
[32] 齐德生.蒙脱石改性前后对AFB₁和营养成分的吸附及对AFB₁的脱毒效果[D].武汉:华中农业大学,2004. QI D S.Adsorption of montmorillonite and modified montmorillonite for AFB₁ and some nutrients and their detoxification for AFB₁ in animals[D].Wuhan:Huazhong Agricultural University,2004.(in Chinese)
[33] 曾路,严春杰,谌刚,等.不同硅酸盐矿物材料对霉菌毒素吸附性能的研究[J].中国畜牧杂志,2011,47(8):64-66. ZENG L,YAN C J,CHEN G,et al.Study on the adsorption properties of different silicate mineral materials for mycotoxins[J].Chinese Journal of Animal Science,2011,47(8):64-66.(in Chinese)

Evaluation of Adsorption Effect of Three Aluminosilicate Adsorbents and Mannooligosaccharides on Five Mycotoxins

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	OU Yunwen, PAN Qin, WANG Yang, DAI Junfei, REN Shaoke, ZHANG Yang, ZHAI Jiajia, ZHANG Jie. Truncated Expression of Cap Protein of Porcine Circovirus Type 3 and Establishment of Indirect ELISA Method for Its Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3056-3066.
[2]	GAO Le, SI Duoduo, GUO Lei, CHEN Can, WANG Wei, WANG Jianlin, WANG Lingling, LI Jidong. Prokaryotic Expression of GA Module-containing Protein in Mycoplasma synoviae and Establishment of Indirect ELISA Method [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2621-2632.
[3]	REN Jianle, LIN Yiting, JI Kang, TAN Shanshan, CHEN Xinxin, JIN Yi, WANG Ying, NIU Sheng, LIANG Libin, LI Junping, ZHAO Yujun, TIAN Wenxia. Preparation of Polyclonal Antibody and Development of an Indirect ELISA Method for Senecavirus A VP2 Protein [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 678-688.
[4]	YANG Dawei, LIU Shuyi, LIN Yating, CHEN Hu, ZHANG Hongliang, LI Guimei. Preparation of Monoclonal Antibody Against gB Protein of Porcine Pseudorabies Virus and Development of a Competitive ELISA Detection Method [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(12): 5439-5448.
[5]	ZHANG Yichen, SUN Mingjun, DENG Changshun, DING Jiabo, XU Baoliang, CHEN Lei, QUAN Chunlan, ZHAO Kang, FAN Xuezheng, QIN Tong. Development and Evaluation of an Indirect ELISA Based on Recombinant E2 Protein for Detection of Bovine Viral Diarrhea Virus Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(12): 5459-5469.
[6]	WU Shangjie, LI Jiajia, JIANG Lin, MA Yuehui, DING Jiabo, HE Xiaohong. Genome-wide Association Study of Anti-brucellosis Traits Based on cELISA Detection in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(10): 4191-4199.
[7]	XU Haojun, LIU Ying, MEI Chen, XU Tong, LI Kai, WANG Hongjun. Development of an Indirect ELISA for Antibody Detection Against Ornithobacterium rhinotracheale Based on Outer Membrane Proteins [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(10): 4420-4428.
[8]	WANG Cheng, XIA Yingju, WANG Haidong, LIU Yebing. Prokaryotic Expression of P54 Protein of African Swine Fever Virus and Establishment of an Indirect ELISA Detection Method [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(10): 4440-4449.
[9]	HOU Yufeng, SU Xiaotong, ZHANG Qiuting, SUN Mingjie, CHEN Tiantian, XIE Quanxi, GU Wei, WANG Hong. Prokaryotic Expression of EtMIC2 and SO7 Proteins of Eimeria tenella and Establishment of an Indirect ELISA Method for Detection of SIgA Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3325-3335.
[10]	ZHANG Fangyuan, LIN Yating, YANG Dawei, CHEN Hu, LI Guimei, SHAN Hu. Prokaryotic Expression of African Swine Fever Virus P30 Protein and Establishment of Indirect ELISA Detection Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2012-2022.
[11]	GUO Hongwei, CAI Yongchao, FAN Chunguang, HAO Kaixuan, CHANG Juan, WANG Ping, LIU Chaoqi, YIN Qingqiang. Effect of Compound Biological Detoxifier on Aflatoxin B₁ Degradation Rate Under Artificial Gastrointestinal Fluid Condition [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1395-1403.
[12]	MU Yijiao, YU Ruiming, ZHANG Liping, WANG Yonglu. Establishment and Application of ELISA for Detection of IgA Antibody Based on the Recombinant Full-length S2 Protein of Porcine Epidemic Diarrhea Virus Variant Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1185-1194.
[13]	NIU Xiaojie, XU Mingguo, XIAO Li, LIU Wenhao, CHEN Chuangfu, WANG Yong. Expression and Purification of IPAJ Protein of Salmonella Pullorum, Preparation of Polyclonal Antibody and Establishment of ELISA Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1218-1228.
[14]	ZHANG Yan, ZHAN Liyuan, JIANG Fujie, MA Hongxia, KONG Lingcong. Prokaryotic Expression, Polyclonal Antibody Production and Development of an Indirect ELISA Antibody Detection Method of Recombinant Protein P48 of Mycoplasma bovis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4621-4631.
[15]	ZHANG Kai, ZHANG Chunping, GE Shengqiang, SUN Chunxi, CHENG Jie, FU Chunyu, WANG Gang, NIU Xing, PENG Jun. Expression of p22 Protein of African Swine Fever Virus,Preparation of Polyclonal Antibody and Establishment of Indirect ELISA Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 270-279.