广东省养鸽场腹泻鸽群沙门菌分离鉴定与耐药性研究

doi:10.16431/j.cnki.1671-7236.2023.12.032

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the prevalence and antimicrobial resistance characteristics of Salmonella in a certain pigeon farm in Guangdong province.【Method】 In this study,diseased pigeons with diarrhea were collected from the pigeon farm for dissection,and Salmonella was isolated and identified from the sample.Serotype identification was performed on the strains identified as Salmonella,and the sensitivity of the isolated strains to 13 antimicrobial drugs was determined using agar dilution method.Furthermore,resistance and virulence genes were tested on the isolated strains.【Result】 A total of 27 strains of Salmonella were isolated from the samples collected from the pigeon farm,with a isolation rate of 67.50% (27/40).The serotype identification results showed that all isolated strains were Salmonella Typhimurium.The drug sensitivity test results showed that the resistance rate of the isolated strains to naphthyric acid and sulfamethoxazole was 100.00%.The resistance rate to streptomycin,tetracycline,and tigecycline was 25.93%.The resistance rate to ampicillin was 11.11% and the resistance rate to flufenicol and chloramphenicol was 3.70%.37.04% of the isolated strains showed multiple drug resistance,and resistance to tigecycline was detected.The results of drug resistance gene testing showed that a total of 5 drug resistance genes could be detected in the isolated strains,among which the detection rates of SulⅡ and tet(A) genes were relatively high,with 29.63% and 25.93%,respectively.The detection rates of bla_TEM,floR and cat1 genes were relatively low,with 11.11%,3.70% and 3.70%,respectively.The results of virulence gene testing showed that the detection rate of mogA,mgtC,bcfA,spvR,spvC,stn and sefA genes was 100.00%,and the detection rate of sseL genes was 96.30%.【Conclusion】 This study successfully isolated 27 strains of Salmonella from pigeons,with severe drug resistance and detection of tigecycline resistant strains.They also carried multiple virulence genes,which might be the main cause of diarrhea in this pigeon population.The results of this study could provide reference for the prevention and control of Salmonella in pigeon farms.

Key words: pigeon; Salmonella; antimicrobial resistance; virulence gene

CLC Number:

S852.61

WU Canji, CHEN Zeluan, ZHENG Zexin, PENG Junhao, HE Haishan, LIANG Yucen, DAI Changzhi, ZHOU Zhouping, LIAO Ming, ZHANG Jianmin. Isolation,Identification and Antimicrobial Resistance Research of Salmonella from Flock of Diarrhea Pigeons in a Pigeon Farm in Guangdong Province[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5104-5113.

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Isolation,Identification and Antimicrobial Resistance Research of Salmonella from Flock of Diarrhea Pigeons in a Pigeon Farm in Guangdong Province

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