冀南地区滑液支原体分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2023.10.041

Abstract

Abstract: 【Objective】 The prevalence and antibiotic resistances of Mycoplasma synoviae (MS) isolates were investigated in the Southern region of Hebei province of China,so as to provide scientific basis for the control of MS infection in this region.【Method】 Samples (the exudate from the airbag,cleft palate,or paw pad of diseased chickens) were collected from 7 large-scale chicken farms suspected of MS infection in the Southern region of Hebei province from 2019 to 2022.Strains were isolated from the samples and identified by PCR.DNA sequence analysis of the vlhA gene was conducted for genotyping of the isolated strains.Minimal inhibitory concentration (MIC) values of the strains were determined by the microbroth dilution method.【Result】 Nine suspected MS strains were isolated from the collected samples.All the strains could turn the liquid culture medium orange and showed typical "fried eggs" colonies on solid culture medium.PCR identification and sequencing results showed that the 9 isolated strains had 100% similarity with the typical HN01 strain,indicating that all of which were MS strains.The vlhA gene typing sequence analysis showed that 9 strains all had 35 amino acis in the proline-rich repeats (PRR),which hence belonged to the L-type,with a nucleotide sequence similarity of 99.7% to 100%.The phylogenetic relationships of isolated strains were close with those L-type strains isolated from other regions of China,whereas far from the L-type strains reported in Thailand.MIC_50/90 values of the 9 MS strains for tylosin,tilmicosin,spiramycin,lincomycin,valnemulin,tiamulin,doxycycline,oxytetracycline,florfenicol,and enrofloxacin were 0.25/0.25,0.5/0.5,0.5/0.5,1/1,≤0.016/≤0.016,0.062/0.125,1/1,2/2,4/4 and 32/32 μg/mL,respectively.【Conclusion】 Nine L-type MS strains were isolated in the Southern region of Hebei province,which showed tiny differences from strains isolated in other regions of China and were relatively stable in genetic evolution.The tiamulin,valnemulin,tylosin,tilmicosin,spiramycin could be recommended for the therapy of MS infections in the region followed by lincomycin,doxycycline,oxytetracycline.The presented data might serve as a guide for the prevention and control of MS infection in the region.

Key words: Mycoplasma synoviae; isolation and identification; vlhA gene; minimum inhibitory concentration

CLC Number:

S852.62

LIU Ziqing, XIONG Weizhen, REN Doudou, ZHOU Shouchang, LIU Guanhui, XU Jinpeng. Isolation,Identification and Drug Resistance Analysis of Mycoplasma synoviae in the Southern Region of Hebei Province[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4270-4278.

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Isolation,Identification and Drug Resistance Analysis of Mycoplasma synoviae in the Southern Region of Hebei Province

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