利用棉绳采集口腔液监测猪伪狂犬病方法的应用分析

doi:10.16431/j.cnki.1671-7236.2021.01.031

Abstract

Abstract: For analyzing the scientific method of using cotton rope to collect oral liquid to monitor pseudorabies (PR) in pig farm,the study was carried out through three aspects,laboratory level,animal test and clinical application.Firstly,the sampling conditions were optimized to determine the ability of the cotton rope to release the Pseudorabies virus (PRV),then the detection time after virus infection was determined by animal experiments,and finally,clinical samples were tested and analyzed.The results showed that it was necessary to suspend a cotton rope with a diameter of 1.0 cm on the pigpen before feeding in the morning,and wait for the pig to bite the cotton rope for 20-30 min.The measurement of virus release ability showed that the release capacity of cotton rope to PRV was about 50%.The measurement of virus release ability showed that 1 TCID₅₀/0.1 mL could be detected.The animal test showed that the pathogenic content in oral fluid was higher than that in nasal swabs except the 5th day in 28 days after infection.And the detection time of the virus (1 d after infection) was earlier than the time when the antibodies turned appear in the blood (7 d after infection).The clinical test results showed that the oral fluid detection method needed to be combined with gE antibody detection to comprehensively determine whether the pig was infected or not,because the vaccine toxicity could also be detected through oral fluids.In summary,this study optimized the oral fluid collection method,and measured the oral fluid release ability of the cotton rope and the detection time after infection,indicating that the oral fluid could be used as a better means of monitoring PR in pigs.In addition,oral fluid detection method needed to be combined with gE antibody detection to comprehensively determine whether the pig herd was an infected group.

Key words: cotton rope; pig; oral liquid; Pseudorabies virus

CLC Number:

S855.3

YU Xuexiang, XU Tuo, HE Dongxian, KU Xugang, LUO Rui, FAN Shengxian, HE Qigai. Application Analysis of the Method for Monitoring Porcine Pseudorabies by Using Cotton Rope to Collect Oral Fluid[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 280-286.

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Application Analysis of the Method for Monitoring Porcine Pseudorabies by Using Cotton Rope to Collect Oral Fluid

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