延边黄牛FGF14基因多态性及其对生长性状的影响

doi:10.16431/j.cnki.1671-7236.2020.12.022

Abstract

Abstract: The purpose of this study was to explore the polymorphism of exon 3 of fibroblast growth factor 14 (FGF14) gene and its effect on growth traits in Yanbian Yellow cattle.The third exon of FGF14 gene was sequenced and analyzed by DNA direct sequencing method,and the genotyping of FGF14 gene was detected by HRM typing technique with high resolution melting curve,so as to explore the relationship between different genotypes of FGF14 gene and growth traits of 16 and 24 months of age in Yanbian Yellow cattle.The results showed that one A>G mutation was detected at position 631 947 bp of FGF14 gene,two alleles of A and G,and three genotypes of AA,AG,and GG.The results of χ² test showed that the distribution of genotype frequency and allele frequency of FGF14 gene in two Yanbian Yellow cattle populations of 16 and 24 months of age conformed to Hardy-Weinberg equilibrium (P>0.05).Polymorphic information content (PIC) analysis results showed that FGF14 gene was moderately polymorphic in both groups (0.25 < PIC < 0.5).The results of association analysis with growth traits showed that the different genotypes of FGF14 gene had significant influence on the traits of height at back and rump length of Yanbian Yellow cattle at 16 months of age (P<0.05),and had significant influence on the traits of body height,body weight and height at hip cross of Yanbian Yellow cattle at 24 months of age (P<0.05).In conclusion,it was speculated that FGF14 gene had a certain influence on the growth and development of Yanbian Yellow cattle,and provided reference for the subsequent modern molecular breeding and breeding of meat traits in Yanbian Yellow cattle.

Key words: Yanbian Yellow cattle; FGF14 gene; SNP; growth traits

CLC Number:

S813.3

WANG Sihan, TIAN Quan, LIU Haixing, JIN Huazi, LI Zhongshu, JI Shuang. Polymorphism of FGF14 Gene and Its Effect on Growth Traits in Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3993-3999.

References

[1] 杜昕桐.延边黄牛产业发展规划研究[D].延吉:延边大学,2019. DU X T.Study on the development planing of Yanbian Yellow cattle industry[D].Yanji:Yanbian University,2019.(in Chinese)
[2] 麻志红.延边黄牛育肥牛不同部位脂肪酸构成与脂肪酸合成相关调节因子的表达研究[D].延吉:延边大学,2017. MA Z H.Fatty acid composition and regulatory factors of fatty acid synthesis in difference parts of fattening beef in Yanbian Yellow cattle the expression of the study[D].Yanji:Yanbian University,2017.(in Chinese)
[3] ARMELIN H A.Pituitary extracts and steroid hormones in the control of 3T3 cell growth[J].Proceedings of the National Academy of Sciences of the United States of America,1973,70(9):2702-2706.
[4] HUI Q,JIN Z,LI X K,et al.FGF family:From drug development to clinical application[J].International Journal of Molecular Sciences,2018,19(7):1875-1890.
[5] 王宝慧,许诺,杨丹,等.FGF14在神经退行疾病中的研究进展[J].中国生物工程杂志,2019,39(1):77-81. WANG B H,XU N,YANG D,et al.Research progress of FGF14 in neurodegenerative diseases[J].China Biotechnology,2019,39(1):77-81.(in Chinese)
[6] 王宝慧.成纤维细胞生长因子14表达及神经保护作用研究[D].温州:温州大学,2019. WANG B H.Expression and biological activity of fibroblast growth factor 14 protein[D].Wenzhou:Wenzhou University,2019.(in Chinese)
[7] WADSWORTH P A,FOLORUNSO O,NGUYEN N,et al.High-throughput screening against protein:Protein interaction interfaces reveals anti-cancer therapeutics as potent modulators of the voltage-gated Na⁽⁺⁾ channel complex[J]. Scientific Reports,2019,9(1):16890.
[8] WANG Z Z,MA H R,XU L,et al.Genome-wide scan identifies selection signatures in Chinese Wagyu cattle using a high-density SNP array[J].Animals,2019,9(6):296.
[9] ABDOLMOHAMMADI A,ZAMANI P.SNP exploring in the middle and terminal regions of the IGF-1 gene and association with production and reproduction traits in Holstein cattle[J].Gene,2014,540(1):92-95.
[10] GUNDERSON K L,STEEMERS F J,LEE G,et al.A genome-wide scalable SNP genotyping assay using microarray technology[J].Nature Genetics, 2005,37(5):549-554.
[11] 国鹏.安格斯牛生长性状相关基因多态性及关联分析[D].银川:宁夏大学,2019. GUO P.Poilmorphism and association analysis of growth traits related genes in Angus cattle[D].Yinchuan:Ningxia University,2019.(in Chinese)
[12] 刘梅.牛羊全基因组拷贝数变异的挖掘及遗传效应研究[D].杨凌:西北农林科技大学,2019. LIU M.Digging of whole genome copy number variation and its genetic effects in cattle and goat[D].Yangling:Northwest A&F University,2019.(in Chinese)
[13] 郭春燕.遗传标记是动物分子育种的基础[J].中国畜牧业,2014,15:52-53. GUO C Y.Genetic markers are the basis of animal molecular breeding[J].China Animal Industry,2014,15:52-53.(in Chinese)
[14] 易恒洁,李辉,赵忠海,等.高坡猪肌肉生长抑制素基因多态性及其与肉质性状的相关性分析[J].中国畜牧兽医,2017,44(4):1102-1107. YI H J,LI H,ZHAO Z H,et al.The polymorphisms of MSTN gene and its relationship with meat quanlity traits in Gaopo pig[J].China Animal Husbandry & Veterinary Medicine,2017,44(4):1102-1107.(in Chinese)
[15] ITOH N,ORNITZ D M.Fibroblast growth factors:From molecular evolution to roles in development,metabolism and disease[J].Journal of Biochemistry, 2011,149(2):121-130.
[16] MEYER M,BEN Y G,RAUSCHENDORFER T,et al.Mouse genetics identifies unique and overlapping functions of fibroblast growth factor receptors in keratinocytes[J].Journal of Cellular and Molecular Medicine,2020,24(2):1774-1785.
[17] HOXHA E,MARCINNO A,MONTAROLO F,et al.Emerging roles of FGF14 in behavioral control[J].Behavioural Brain Research,2019,356:257-265.
[18] 夏天,林仙花,唐业东,等.成纤维细胞生长因子信号通路与小鼠肺发育[J].动物医学进展,2015,36(11):106-109. XIA T,LIN X H,TANG Y D,et al.Signaling pathway of fibroblast growth factors and development of mouse lung[J].Progress in Veterinary Medicine,2015,36(11):106-109.(in Chinese)
[19] 赵高锋.秦川牛和南阳牛生长发育性状的分子标记研究[D].杨凌:西北农林科技大学,2007. ZHAO G F.Study on DNA molecular marker of growth and development traits in Qinchuan cattle and Nanyang cattle[D].Yangling:Northwest A&F University,2017.(in Chinese)
[20] 孟庆杰,黄保荣,陈贤云,等.FGF1基因多态性与2型糖尿病脂代谢的相关性[J].广东医学,2015,36(18):2856-2858. MENG Q J,HUANG B R,CHEN X Y,et al.Correlation between FGF1 gene polymorphism and lipid metabolism in type 2 diabetes[J].Guangdong Medical Journal,2015,36(18):2856-2858.(in Chinese)
[21] 张立春,尹峰,柳俭强,等.绵羊FGF5基因Exon 3多态性对毛用性状的影响[J].中国草食动物科学,2018,38(6):1-5. ZHANG L C,YIN F,LIU J Q,et al.Relationship between polymorphisms of FGF5 gene exon 3 and wool traits[J].China Herbivore Science,2018,38(6):1-5.(in Chinese)
[22] 安清明.绵羊ADIPOQ、UCP1和FGF21基因遗传变异与生长及胴体肌肉性状关联性研究[D].兰州:甘肃农业大学,2016. AN Q M.Effect of ADIPOQ,UCP1 and FGF21 variations on growth and carcass muscle traits in sheep[D].Lanzhou:Gansu Agricultural University,2016.(in Chinese)
[23] SU T H,HUANG L L,ZHANG N,et al.FGF14 functions as a tumor suppressor through inhibiting PI3K/AKT/mTOR pathway in colorectal cancer[J].Journal of Cancer,2020,11(4):819-825.
[24] 张桂山.辽宁绒山羊与乾华肉用美利奴羊皮肤毛囊miRNA筛选及靶基因验证[D].长春:吉林农业大学,2016. ZHANG G S.Screening of miRNAs and identification of candidate target genes in skin and hair follicle from Liaoning cashmere goat and Qianhua mutton merino[D].Changchun:Jilin Agricultural University,2016.(in Chinese)
[25] 谷博.辽宁绒山羊次级毛囊周期性发育相关基因表达的研究[D].长春:吉林农业大学,2013. GU B.Study of expression of genes that is related to periodic development of Liaoning cashmere goat secondary follicle[D].Changchun:Jilin Agricultural University,2016.(in Chinese)
[26] 叶茂,宋志文,金成龙,等.骨骼肌生长发育及再生过程中Wnt信号网络的作用机制[J].动物营养学报,2020,32(8):3560-3567. YE M,SONG Z W,JIN C L,et al.Mechanism of Wnt signaling network in skeletal muscle growth,development and regeneration[J].Chinese Journal of Animal Nutrition,2020,32(8):3560-3567.(in Chinese)
[27] 曹校瑞,高淑媛,牛姝,等.成纤维细胞生长因子FGF11、13、18对小鼠毛囊第一生长周期作用的研究[J].中国兽医杂志,2019,55(3):33-38. CAO X R,GAO S Y,NIU S,et al.Effects of fibroblast growth factors 11,13 and 18 on the first growth cycle of hair follicles in mice[J].Chinese Journal of Veterinary Medicine,2019,55(3):33-38.(in Chinese)
[28] 路宏朝.FGF13影响小鼠骨骼肌增殖与分化的机制研究[D].杨凌:西北农林科技大学,2016. LU H C.The effects of FGF13 in skelatal muscle proliferation and differention in mice[D].Yangling:Northwest A&F University,2016.(in Chinese)
[29] 张力青,付平,易四凤,等.成纤维细胞生长因子(FGF)在骨骼肌发育中的作用[J].养殖与饲料,2016,1:6-10. ZHANG L Q,FU P,YI S F,et al.Role of fibroblast growth factor (FGF) in skeletal muscle development[J].Animals Breeding and Feed,2016,1:6-10.(in Chinese)
[30] LIN H P,LU P Y,ZHOU M,et al.Purification of recombinant human fibroblast growth factor 13 in E.coli and its molecular mechanism of mitogenesis[J].Applied Microbiology and Biotechnology,2019,103(17):7017-7027.

Polymorphism of FGF14 Gene and Its Effect on Growth Traits in Yanbian Yellow Cattle

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[2]	LI Mingbo, GAO Qingshan, LI Guanhao, ZHANG Kui, CUI Lianhua, HOU Lina. Effects of Chinese Herbal Medicine Feed Additives on Meat Quality and Flavor Substances in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 531-542.
[3]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[4]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[5]	FENG Xiaopin, LI Yinxia, ZHANG Chenjian, ZHANG Jun, MENG Chunhua, QIAN Yong, CAO Shaoxian. Coning and Polymorphism Identification of PLAG1 Gene and Its Association Analysis with Birth Weight and Body Size Traits in Boer Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3465-3474.
[6]	YANG Tao, ZHANG Mingjie, XU Ran, ZHANG Han, ZHANG Mengmeng, WU Keliang. Study on Genetic Evaluation of Reproductive and Growth Traits in Pigs by BLUP Method [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2971-2981.
[7]	ZHOU Ya, ZHANG Heyang, LIU Linling, LI Haodong, ZHENG Junjun, WANG Guiwu. Analysis of FGFR2 Gene Polymorphism and Their Correlation with Antler Weight Traits in Sika Deer [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3006-3014.
[8]	QIANG Hao, LIANG Peng, MENG Ke, RONG Xuan, FENG Dengzhen. Polymorphism of IGF1R Gene and Its Association Analysis with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1765-1785.
[9]	LI Jingyun, WANG Xinle, ZHANG Yunfei, BAI Junyan, LI Gan, LEI Ying, DONG Zhihao, CHEN Yu, FAN Hongdeng, WANG Longwei, CHEN Mengke, WANG Xingke, SIMA Leiyang, ZHANG Shiwen. Polymorphism of MyoG Gene Intron Ⅱ and Its Association with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1364-1373.
[10]	RUAN Yong, CHEN Xiang, DAI Lingang, HUANG Jiajin, AN Dongwei, ZOU Qishun. Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1384-1392.
[11]	DENG Yihua, WANG Tianjiao, WANG Hongliang, DONG Yimeng, LIU Xin, XING Xiumei. Screening of Specific SNP Sites in Tahe Red Deer Based on Resequencing Technology [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1413-1421.
[12]	JIANG Hongzheng, YANG Dezhi, MA Zhonghua, XUN Wenjuan, SHI Liguang, HOU Guanyu. Genome-wide Association Study of Body Size Traits in Danzhou Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 598-607.
[13]	WANG Congliang, FU Qi, JU Minying, BAI Jingjing, LI Xinshu, SONG Xiaoyue, LI Longping, LAN Xianyong, QU Lei, ZHANG Lei, ZHU Haijing. The Genetic Polymorphisms of KAP9.2 Gene and Its Correlation Analysis with Growth Traits of Inner Mongolia Cashmere Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4270-4278.
[14]	ZHOU Ya, ZHANG Heyang, LIU Linling, LI Haodong, ZHENG Junjun, WANG Guiwu. Polymorphism of MSRB3 Gene and Its Association Analysis with Antler Weight Traits in Sika Deer [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4279-4287.
[15]	FENG Mian, SHANG Mingyu, BI Yazhen, HU Wenping, ZHANG Li. Polymorphisms of FUT8 Gene and Its Association Analysis with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3838-3845.