miR-93对延边黄牛垂体细胞GH分泌的影响

doi:10.16431/j.cnki.1671-7236.2020.01.014

Abstract

Abstract: In order to analyze the effect of miRNAs on growth hormone (GH) secretion in pituitary cells of Yanbian Yellow cattle,the differently expressed miR-93 in the blood of Yanbian Yellow cattle and Hanyan cattle was selected to analyze the effects of miR-93 on GH secretion in pituitary cells of Yanbian Yellow cattle.The primary culture of Yanbian Yellow cattle pituitary cells was carried out,and mimics (miR-93-mi group),mimics reference substance (NC control group),inhibitor (miR-93-in group) and inhibitor reference substance (iNC group) of miR-93 were transfected into the established pituitary primary cells.After 48 h,the cells were collected and the total RNA and protein were extracted.The target genes of miR-93 were predicted by targetscan and RNA hybrid analysis software,and the target relationship of miR-93 was validated by double luciferase reporter gene system.The GH mRNA transcription and protein expression were detected by Real-time PCR and Western blotting,respectively.The results showed that miR-93 targeted 3'UTR of growth hormone releasing hormone receptor (GHRHR);The transcription and protein expression of GH in pituitary cells of Yanbian Yellow cattle in miR-93-mi group were extremely significantly lower than those of NC control group (P<0.01);The mRNA transcription and protein expression of GHRHR in miR-93-mi group were extremely significantly lower than those of NC control group (P<0.01),while the protein expression of GHRHR in miR-93-in group were significantly higher than those of iNC group (P<0.05).The above results demonstrated that miR-93 could regulate GH secretion in pituitary cells by regulating the expression of GHRHR gene and further regulated the growth and development of Yanbian Yellow cattle.

Key words: miR-93; Yanbian Yellow cattle; pituitary cells; GH; GHRHR

CLC Number:

S823.8

LOU Angang, CUI Changyan, XIANG Siyu, JIN Taihua, CHEN Yingying, ZHANG Rui, GUAN Lizeng. Effect of miR-93 on GH Secretion in Pituitary Cells of Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 116-123.

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Effect of miR-93 on GH Secretion in Pituitary Cells of Yanbian Yellow Cattle

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