氟苯尼考胁迫下弗氏柠檬酸杆菌转录组测序研究

doi:10.16431/j.cnki.1671-7236.2019.11.005

Abstract

Abstract: In order to explore the resistance mechanism of Citrobacter freundii,florfenicol was used as an inducer,RNA was extracted from normal cultured strains and strains under flufenicol stress at 400 μg/mL.Gene expression levels and differentially expressed genes between strains were analyzed by RNA sequencing (RNA-Seq).The results showed that the average output of each sample was 1.31 Gb.The mean alignment rate with reference genome was 67.01%.Through the detection of differentially expressed genes among samples,a total of 2 019 significantly differentially expressed genes were screened,among which 963 genes were up-regulated and 1 056 genes were down-regulated,and 2 genes of emrA and emrB were found.KEGG Pathway analysis showed that the most differentially expressed genes were enriched in metabolism,accounting for 72% of the total.Carbohydrate metabolism,energy metabolism,amino acid metabolism,membrane transport and other pathways were significantly enriched.GO functional analysis showed that the distribution of significantly different genes in biological processes was the largest,accounting for 46% of all genes.Significant differentially expressed genes mainly involved metabolic process,catalytic activity and binding.It was suggested that the resistance of Citrobacter freundii might be related to active efflux mediated by extracellular pump,target change,enzyme hydrolysis,membrane permeability change and biofilm formation.

Key words: Citrobacter fredii; fluphenicol; RNA-Seq; drug resistance

CLC Number:

S852.61

WEI Panqiu, HUANG Jie, HAN Shuyu, LUO Fuguang, LI Ya, YI Yi. RNA Sequencing of Citrobacter fredii Under Fluphenicol Stress[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3172-3180.

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RNA Sequencing of Citrobacter fredii Under Fluphenicol Stress

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