一例鸭疫里默氏杆菌病的诊断及病原分离鉴定

doi:10.16431/j.cnki.1671-7236.2019.04.026

Abstract

Abstract:

In order to diagnose the pathogen which caused disease of one duck farm in Shangqiu in Henan province,the brain,liver,spleen,and lungs of diseased ducks were collected,and AIV-H9,DTMUV,DRV and NDV were tested according to PCR detection methods of nucleic acid.Isolation and identification of bacteria was operated by serum-agar plates and conventional PCR detection methods.The serotype of the isolated strain was identified by glass agglutination,and the animal regression test was conducted.In order to screen sensitive drugs of the pathogen,31 kinds of antibacterials were chosen in the drug sensitivity test.The results showed that the detections of AIV-H9,TMUV,DRV and NDV were negative,which means these four viral diseases was initially excluded.The liver and brain tissues were cultured in serum nutrient AGAR medium aseptically and there were a translucent colonies at the size of a needle on culture medium.PCR amplification of purified culture broth showed that 338 bp specific bands of RA were amplified which was identified as RA serotype 7.The animal regression test showed that the isolated strain could be successfully replicated in clinical practice and could show obvious clinical infection symptoms of infectious serositis.The drug sensitivity test results showed that the isolates were sensitive to cefixime,cefazolin,cefradine,ceftriaxone,spectinomycin,doxycycline,levofloxacin,florfenicol,lincomycin and ampicillin (sulbactam).In conclusion,the pathogen causing the disease in the duck farm was Riemerella anatipestifer serotype 7 and some antibacterials could be used such as cephalosporin antibiotics.

Key words: Riemerella anatipestifer (RA); isolation and identification; drug sensitivity test; serotypes; animal regression test

CLC Number:

S852.61

HAO Dongmin, ZHONG Hongyi, CHENG Binghua, LU Ailing, XU Chuanbao, MA Youquan. Diagnosis of Riemerella anatipestifer Infection and Identification of the Pathogens[J]. , 2019, 46(4): 1174-1180.

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Diagnosis of Riemerella anatipestifer Infection and Identification of the Pathogens

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