1株多重耐药致病性大肠杆菌的分离鉴定与分子分群

doi:10.16431/j.cnki.1671-7236.2019.04.020

Abstract

Abstract:

In January 2017,a diarrhea epidemic occurred in calves of a cattle farm in Bole,Xinjiang.The purpose of this study was to investigate the etiology and formulate corresponding prevention and control measures.Anal swabs and stool samples from 6 diarrhoeal calves were collected,rotavirus,coronavirus,Cryptosporidium and Escherichia coli F5 (K99) were detected by Speed V-Diar 4^TM test paper.Then the samples were isolated,identified and molecular clustering,the pathogenicity and drug resistance of the isolated bacteria were detected.The test results of all stool samples were negative.6 strains of Escherichia coli and 1 strain of Edwardsiella tarda were obtained from 6 samples.Phylogenetic PCR analysis showed that 6 strains of Escherichia coli A group accounted for 83.3%(5/6),F group accounted for 16.7%(1/6).Half the lethal dose result showed that only one strain of Escherichia coli (strain XJ-B1) was pathogenic,and could cause diarrhea and death in mice.The results of drug sensitivity test showed that 7 isolates had different degrees of resistance to the 18 antibiotics,and Escherichia coli XJ-B1 was the most serious drug-resistant strain.It was multidrug-resistant to beta-lactams,sulfonamides and polypeptide antibiotics.Therefore,this pathogenic Escherichia coli might be one of the causes of this diarrhea in calves,and it was also the first group F Escherichia coli isolated from cattle.The multi-drug resistance of the bacteria increased the difficulty of clinical treatment.This results provided a basis for the cattle farm to treat the calf diarrhea.

Key words: calves; diarrhea; phylogenetic group; pathogenicity; drug sensitivity test

CLC Number:

S852.61⁺2

SU Zhanqiang, MA Kaiqi, TONG Panpan, WANG Dong, GAO Jiaojiao, SUN Xue, CHEN Rulong, YAO Gang. Isolation,Identification and Phylogenetic Group of a Strain of Multidrug-resistant Pathogenic Escherichia coli[J]. , 2019, 46(4): 1127-1134.

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Isolation,Identification and Phylogenetic Group of a Strain of Multidrug-resistant Pathogenic Escherichia coli

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