貉早期胚胎发育内环境的研究

doi:10.16431/j.cnki.1671-7236.2019.02.017

Abstract

Abstract:

To investigate the micro-environment in vivo of preimplantation embryos in the raccoon dog,23 adult healthy females with the same age and similar body weight were selected.After 1-2 times of copulation during breeding season,starting with the first mating(0 h),the females were killed randomly at 29-99 h (n=7),100-126 h(n=7),169-268 h (n=9),respectively.The morphological and ultrastructural changes of the oviduct and uterus during early embryonic development were analyzed using immunohistochemistry,transmission and scanning electron microscopy.9 elements such as potassium,calcium,iron,zinc of sputum oviduct fluid and uterus fluid during early embryo development were determined by X-ray energy spectroscopy.The results showed that:① With the development of early embryos of raccoon dog,at the later stage,the length,mucosal thickness,duplicatures' height and epithelial height of oviduct were significantly reduced (P<0.05);Although the diameter of oviduct was also decreased,but there was no significant difference (P>0.05).On the other hand,the mucosal thickness,duplicatures' height and density of uterine gland were significantly increased (P<0.05);Although the diameter and length of uterus were enhanced,but the difference was not significant (P>0.05).②With the development of early embryos of raccoon dog,the numbers of microvilli,lipid droplets and lysosomes in the uterine mucosa epithelium were magically increased.③With the development of early embryos of raccoon dog,at the later stage,the contents of S,Ca,Fe,Cu and Zn in the oviduct fluids were significantly improved (P<0.05),while the content of P in the oviduct fluids was significantly decreased (P<0.05),the contents of K,Cl and Na in the oviduct fluids showed the fluctuation change with the development of embryos.The contents of S,Cl,K in the uterus fluids were decreased (P>0.05),the content of Zn in the uterus fluids was significantly decreased (P<0.05),the contents of P,Ca,Fe and Cu presented the fluctuation change with the development of embryos.This study preliminarily investigated the physical and chemical micro-environment in vivo of preimplantation embryos in raccoon dogs,which would provide reference for the establishment of culture system of preimplantation embryos in vitro in raccoon dogs.

Key words: raccoon dog; oviduct; uterus; embryos

CLC Number:

S865.2

CAO Junguo, DUAN Baoning, XU Baozeng, FENG Huailiang. Study on Inner Environment of Early Embryos Development in Racoon Dog[J]. , 2019, 46(2): 470-479.

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Study on Inner Environment of Early Embryos Development in Racoon Dog

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