北极狐及乌苏里貉抑制素α亚基基因克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2017.12.001

Abstract

Abstract:

This study was aimed to clone the inhibin α(INHα) subunits gene of arctic fox and Wusuli raccoon dog and analyze its genetic structure with bioinformatics. According to the published sequence of INHα subunit gene (GenBank accession No. XM_545660.5), one pair of specific PCR primers were designed. The INHα subunit gene of arctic fox and Wusuli raccoon dog were amplified by RT-PCR. The target gene was linked into cloning vector to be sequenced and systemically analyzed by bioinformatics. The results showed that INHα subunit gene fragment of arctic fox and Wusuli raccoon dog included an 1 107 bp whole length CDS (encoding 369 amino acids). The INHα subunit gene of arctic fox and Wusuli raccoon dog shared 97.9% and 97.6% of similar nucleotide sequence with INHα subunit gene of dog, respectively. Phylogenetic analysis showed that the INHα subunit gene of arctic fox and Wusuli raccoon dog were genetically close to dog, and it was highly conserved in different species and evolutionary processes. The prediction of INHα subunit tertiary structure showed that the cysteine knot directs members of the INHα subunit to adopt a "butterfly-shaped" or "open hand" configuration, with the α-helix defining the "wrist" and β-sheets forming the "fingers" of the molecule. The INHα subunit gene of arctic fox and Wusuli raccoon dog were cloned successfully and analyzed by bioinformatics software, which would help to research the biological function of inhibin in the simultaneous development of oocyte-granulosa cells.

Key words: arctic fox; Wusuli raccoon dog; INHα subunit gene; cloning; bioinformatics analysis

CLC Number:

ZHANG Yu-fei, LI Xiao-xia, WANG Shi-yong, CAO Xin-yan, DIAO Yun-fei, YANG Yi-feng, CHEN Xiu-min, ZHAO Wei-gang, ZHAO Meng, WEI Hai-jun, XU Bao-zeng. Cloning and Bioinformatics Analysis of Inhibin α-subunit Gene of Arctic Fox and Wusuli Raccoon Dog[J]. , 2017, 44(12): 3391-3400.

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Cloning and Bioinformatics Analysis of Inhibin α-subunit Gene of Arctic Fox and Wusuli Raccoon Dog

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