黑貉TYRP1基因的克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2019.05.006

Abstract

Abstract:

To identify CDS sequence of tyrosinase-related protein 1 (TYRP1) gene in Black raccoon dog and perform bioinformatics analysis,the back skin tissue of Black raccoon dog was collected to extract RNA in this study,and RNA was reverse transcribed into cDNA.One pair of specific primers was designed according to the canine TYRP1 gene mRNA sequence (accession No.:NM_001194966.1) in GenBank.The TYRP1 gene CDS was amplified by RT-PCR,PCR products were inserted into the cloning vector,and verified by positive colony PCR and sequenced.The results showed that the CDS sequence of TYRP1 gene in Black raccoon dog was 1 614 bp in length and encoded 537 amino acids,the TYRP1 protein was an unstable soluble hydrophilic protein.The genetic distance was closer between Black raccoon dog and fox,indicating that TYRP1 gene was highly conserved in different species and evolution processes.The proportion of random coil in the secondary structures of TYRP1 protein was the highest (54.0%),followed by alpha helix,which was 29.8%.The tertiary structure of TYRP1 protein was mainly composed of a large number of random coil and alpha helix,which was consistent with the secondary structure.TYRP1 protein had oxidoreductase activity,which was involved in DNA repair and replication,protein synthesis and degradation,and interacted with various proteins such as TYR,DCT,TP53 and TRPC1.The results of this experiment provided a theoretical basis for further exploration of its function in mammalian hair color genetic mechanism of TYRP1 gene in Black raccoon dog.

Key words: Black raccoon dog; TYRP1 gene; cloning; bioinformatics analysis

CLC Number:

Q785

GUO Yueyue, ZHANG Yufei, WANG Tianjiao, XING Xiumei, XU Chao, ZHAO Jiaping. Cloning and Bioinformatics Analysis of TYRP1 Gene in Black Raccoon Dog[J]. , 2019, 46(5): 1299-1307.

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Cloning and Bioinformatics Analysis of TYRP1 Gene in Black Raccoon Dog

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