表达猪乳铁蛋白肽重组屎肠球菌对断奶仔猪生长性能的影响及其抗ETEC感染效果研究

doi:10.16431/j.cnki.1671-7236.2018.12.013

Abstract

Abstract:

In this study,the effects of recombinant Enterococcus faecium expressing porcine lactoferrin peptide (pNZ8112-PLFcin/Ef) on growth performance and its protection activity aganist enterotoxigenic Escherichia coli (ETEC) of weaned piglets were examined.36 healthy weaned piglets of 28 days old with similar body weight were selected and randomly divided into 3 groups with 3 replicates per group and 4 piglets per replicate.The piglets in recombinant Enterococcus faecium group was fed the basal diet supplemented with 6×10¹² CFU/kg pNZ8112-PLFcin/Ef,that in pNZ8112/Ef group was fed basal diet supplemented with 6×10¹² CFU/kg pNZ8112/Ef,while that in the medium group was fed basal diet supplemented with the same volume of GM17 medium.The experiment lasted for 26 d.The results showed that compared with medium group,the average daily gain of weaned piglets in the recombinant Enterococcus faecium group was extremely significantly increased (P<0.01),the F/G was significantly decreased (P<0.05).And the occurrence of piglet diarrhea was reduced to a certain extent.The evenness and diversity indexes of intestinal flora in weaned piglets were decreased.In order to further explore the antibacterial activity of pNZ8112-PLFcin/Ef,the protective effect of ETEC infection was explored in this study.After continuous feeding for 21 days,one piglet randomly selected from each replicate and fed with ETEC.The results showed that compared with the medium group,the levels of IL-2 and IgG in serum,sIgA in intestinal mucus and spleen index of recombinant Enterococcus faecium group were significantly increased (P<0.05),but there was no significant difference in thymus index and the length and weight of small intestinal (P>0.05).In conclusion,the studies had shown that recombinant Enterococcus faecium expressing porcine lactoferrin could promote the growth of piglets and had protective effect of ETEC infection.

Key words: porcine lactoferricin; recombinant Enterococcus faecium; weaned piglets; enterotoxigenic Escherichia coli(ETEC)

CLC Number:

S828

XIE Weichun, WANG Yufeng, PI Ruobing, ZHAO Dongfang, JIANG Yanping, CUI Wen, QIAO Xinyuan, WANG Li, ZHOU Han, XU Yigang, LI Yijing, TANG Lijie. Effect of Recombinant Enterococcus faecium Expressing Porcine Lactoferrin Peptide on Growth Performances and Its Protection Activity Aganist ETEC of Weaned Piglets[J]. , 2018, 45(12): 3408-3418.

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Effect of Recombinant Enterococcus faecium Expressing Porcine Lactoferrin Peptide on Growth Performances and Its Protection Activity Aganist ETEC of Weaned Piglets

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