山羊单增李斯特杆菌病的诊断与防治

doi:10.16431/j.cnki.1671-7236.2018.10.034

Abstract

Abstract:

The goats in a farm in Western Jiangxi showed neurological symptoms such as circling movements,unilateral blindness and dyspnea,nearly 20 goats died.In order to identify the pathogens and establish effective prevention and treatment measures,the pathological necropsy was performed on three dead sheep that were sent for inspection.The viscera tissues such as liver,spleen and brain tissues were collected for the purification and cultivation of pathogenic bacteria.And the LB medium was used to make a secondary enrichment isolation selectively.Gram staining,biochemical tests,and 16S rRNA gene sequencing analysis were carried out.The animal experiments in mice and rabbits were conducted in order to identify the pathogenicity of pathogenic bacteria.To identify the sensitivity of pathogenic bacteria to antibacterial drugs,cefazolin,ceftriaxone sodium,penicillin,vancomycin and other 30 antibacterial agents were used in bacteriostasis experiment in vitro of pathogenic bacteria.The results were as follows:Two strains of Gram-positive bacteria were isolated from the brain tissue of two sheep,both of which were identified as Listeria monocytogenes with strong virulence.The homology between two strains was 100.0%,and that of two strains and other referenced Listeria were higher than 99.0%.The two isolates were highly sensitive to drugs of cefazolin,piperacillin,carbenicillin,tetracyclines,doxycycline,aboren,erythromycin,vancomycin and chloroamphenicol.However,they were resistant to the 1st and 2nd generation cephalosporin antibiotics,oxacillin and PCST.This farm should adopt comprehensive prevention and control measures according to drug sensitivity test results to better control the disease in order to keep the safety of goats and meat products.

Key words: goat; listeriosis; Listeria monocytogenes; drug susceptibility test; 16S rRNA

CLC Number:

S852.61

TANG Mude, WEN Yuting, WU Qingqing, AYIJIANG·ABITI, WU Gang, KASSIM ALI BAKAR, DENG Qiuchun, LIU Ben, ZHENG Wenya. Diagnosis and Treatment of Goat Listeriosis[J]. , 2018, 45(10): 2951-2959.

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Diagnosis and Treatment of Goat Listeriosis

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