1例鸡白痢沙门氏菌的分离鉴定与耐药性分析

doi:10.16431/j.cnki.1671-7236.2018.09.037

Abstract

Abstract:

The study was aimed to investigate the pathogens of a suspected Salmonella infection from a large-scale layer farm in Xinxiang city Henan province,and provide a reasonable treatment.The intestinal tube samples of sick chickens were collected under sterile condition,and gram staining,culture characteristics,biochemical test and PCR were carried out,the pathogen regression was performed,also,the drug sensitive of 19 kinds of common antibiotics were analyzed using the disk diffusion (K-B) method.The results showed that a strain of gram-negative bacillus was successfully isolated,and consistent with the culture characteristics and pathologic characteristics of Salmonella Pullorum,the invA gene was amplified successfully by PCR,and the strain was identified as Salmonella Pullorum by comparison with the GenBank database;Using isolated bacterial strain to infect SPF chickens could replicate cases consistent with natural infections.It indicated that the Salmonella Pullorum was the main pathogenic agent of the chickens in this chicken farm.The pathogenic and multiple drug resistance of the Salmonella Pullorum were strong.The isolate strain was resistant to amikacin,oxacillin and penicillin,but it was sensitive to fortum (ceftazidime),ampicillin and ceftriaxone,which could be recommended for clinical treatment positive effect.Above all,this study proposed the specific prevention and treatment measures for the disease and achieved good results.This study initially provided a reference for the diagnosis and treatment of Salmonella Pullorum,with great clinical significance in the early diagnosis and treatment of pathogens of Salmonella Pullorum.

Key words: Salmonella Pullorum; isolation and identification; drug sensitivity test

CLC Number:

S852.61

DONG Mengmeng, WANG Xiaofei, XU Yanzhao, WANG Qing, SUN Yawei, HANG Bolin, HU Jianhe. Isolation, Identification and Analysis of Drug Resistance of Salmonella Pullorum[J]. , 2018, 45(9): 2637-2644.

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Isolation, Identification and Analysis of Drug Resistance of Salmonella Pullorum

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