一株牛源乡间布丘菌的分离鉴定及耐药性研究

doi:10.16431/j.cnki.1671-7236.2018.08.036

Abstract

Abstract:

To understand the biological characteristics and resistance of pathogenic microorganisms of dairy cows with mastitis,a gram-negative bacterium,named HLJ-36,was isolated from dairy cow's mastitis milk.Morphological characteristics,biochemical identification,molecular biological identification,mouse pathogenicity test and drug susceptibility test were performed.Biochemical test results showed that xylose,ONPG,citrate and so on were positive;Oxidase,sputum,urease and so on were negative.The physicochemical characteristics and molecular biological test results showed that the isolate was consistent with the characteristics of Buttiauxella agrestis,and had the highest homology with human Buttiauxella agrestis (GenBank accession No.:NR_041968.1) isolated from intestine,reaching 99%.The pathogenicity test in mice showed that mice in the experimental group did not die within 72 hours,indicating that the isolate was less pathogenic.Drug susceptibility test results showed that the bacteria were resistant to ceftiofur,azithromycin,ciprofloxacin,etc.,and sensitive to cefoxitin,meropenem,chloramphenicol,amoxicillin-clavulanic acid,and gentamicin.The results showed that Buttiauxella agrestis might be a pathogen causing dairy cow mastitis and had a certain degree of drug resistance,due to the highest homology of this strain and human Buttiauxella agrestis (GenBank accession No.:NR_041968.1),there was a possibility of transmission to humans through milk and dairy products,which had certain public health significance.

Key words: Buttiauxella agrestis; isolation and identification; drug sensitive test

CLC Number:

S855.1

DU Lin, WANG Lifang, FENG Xiaohui, ZHANG Sanfen, SONG Jie, YAO Yiping, SHI Pei. Isolation, Identification and Drug Resistance Analysis of a Bovine Buttiauxella agrestis[J]. , 2018, 45(8): 2334-2340.

References

[1] 刘琪,王秋东,崔彪,等.内蒙古部分地区致奶牛乳房炎链球菌的流行病学及生物学特性分析[J].中国预防兽医学报,2017,39(11):875-879.LIU Q,WANG Q D,CUI B,et al.Molecular epidemiology and characteristics of Streptococcus of dairy cow in Inner Mongolia area[J].Chinese Journal of Preventive Veterinary Medicine,2017,39(11):875-879.(in Chinese)
[2] 姚伟,高锋,曹明慧,等.辽宁地区奶牛乳房炎源大肠杆菌毒力基因及耐药基因检测分析[J].中国畜牧兽医,2017,44(6):1832-1839.YAO W,GAO F,CAO M H,et al.Detection and analysis of virulence genes and drug resistance genes in E.coli strains isolated from dairy cows with clinical mastitis in Liaoning region[J].China Animal Husbandry & Veterinary Medicine,2017,44(6):1832-1839.(in Chinese)
[3] PELLEGRINO M,BERARDO N,GIRAUDO J,et al.Bovine mastitis prevention:Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period[J].Beneficial Microbes,2017,30(3):1-8.
[4] TURK R.The role of oxidative stress and inflammatory response in the pathogenesis of mastitis in dairy cows[J].Mljekarstvo,2017,67(2):91-101.
[5] KOSE F,OZCAN E,SONMEZ K,et al.A rare cause of neonatal breast abscess and mastitis:Foeniculum vulgare and power full massage[J]. Hong Kong Journal of Paediatrics,2016,21(2):1-4.
[6] KACZOREK E,MALACZEWSKA J,WÍJCIK R,et al.Biofilm production and other virulence factors in Streptococcus spp.isolated from clinical cases of bovine mastitis in Poland[J].Bmc Veterinary Research,2017,13(1):398.
[7] KSOURI S,DJEBIR S,HADEF Y,et al.Survey of bovine mycotic mastitis in different mammary gland statuses in two North-Eastern regions of Algeria[J]. Mycopathologia,2015,179(3-4):327-331.
[8] NICHOLAS R A,FOX L K,LYSNYANSKY I.Mycoplasma mastitis in cattle:To cull or not to cull[J].Veterinary Journal,2016,21(6):142-147.
[9] TOUSSAINT A,SIMONSON C,VALLA C.Herpes mastitis:Diagnosis and management[J]. Breast Journal,2016,22(3):335-338.
[10] FERRAGUT C,IZARD D,GAVINI F,et al.Buttiauxella,a new genus of the family Enterobacter-aceae[J].Zentralblatt Für Bakteriologie Und Hygiene Reihe C, 1981,2(1):33-44.
[11] 魏民,周敏,朱叶秋,等.乡间布丘菌致败血症一例[J].中华医学检验杂志,1999,22(2):54.WEI M,ZHOU M,ZHU Y Q,et al.A case of bacterial sepsis caused by Buttiauxella agrestis[J] Chinese Journal of Laboratory Medicine,1999,22(2):54.(in Chinese)
[12] ANTONELLO V S,DALLÉ J,DOMINGUES G C,et al.Post-cesarean surgical site infection due to Buttiauxella agrestis[J].International Journal of Infectious Diseases,2014,22(5):65-66.
[13] MVLLER H E,BRENNER D J,FANNING G R,et al.Emended description of Buttiauxella agrestis with recognition of six new species of Buttiauxella and two new species of Kluyvera:Buttiauxella ferragutiae sp.nov.,Buttiauxella gaviniae sp.nov.,Buttiauxella brennerae sp.nov., Buttiauxella izardii sp.nov.,Buttiauxella noackiae sp.nov., Buttiauxella warmboldiae sp.nov.,Kluyvera cochleae sp.nov.,and Kluyvera georgiana sp.nov[J].International Journal of Systematic Bacteriology,1996,46(1):50-63.
[14] DEVI L,MEIGNANALAKSHMI S,SWORNA K C,et al.Isolation and characterization of PHB producing Buttiauxilla agrestis from slaughter house waste rumen fluid[J].International Journal of Scientific Research,2015,4(6):782-785.
[15] BAERE T D,WAUTERS G,KÄMPFER P,et al.Isolation of Buttiauxella gaviniae from a spinal cord patient with urinary bladder pathology[J]. Journal of Clinical Microbiology,2002,40(10):3867-3870.
[16] MARDARE T,GHIBU F,VARGANICI C.Isolation and characterization of fermentative bacteria from the intestine of two edible snails,helix pomatia and Cantareus aspersus (Gastropoda:Pulmonata)[J].Kardiologiia,2006,53(2):1-5.
[17] PARK J W,OH Y S,LIM J Y,et al.Isolation and characterization of cold-adapted strains producing beta-galactosidase[J].Journal of Microbiology,2006,44(4):396-402.
[18] SPRÖER C,MENDROCK U,SWIDERSKI J,et al.The phylogenetic position of Serratia,Buttiauxella and some other genera of the family Enterobacteriaceae[J].International Journal of Systematic Bacteriology,1999,49(4):1433-1438.
[19] 伍好.肉鸡源性肠杆菌科细菌中耐药基因、整合子及ISCR1元件的研究[D].济南:山东大学,2016.WU H.The research on the antibiotic resistance genes,integrons and ISCR1 elements among Enterobacteriaceae from chicken broilers[D].Jinan:Shandong University,2016.(in Chinese)
[20] 翁幸鐾,糜祖煌.可移动遗传元件:耐药基因的载体[J].中国人兽共患病学报,2013,29(4):389-397.WENG X B,MI Z H.Mobile genetic elements:Carrier of resistance genes[J].Chinese Journal of Zoonoses,2013,29(4):389-397.(in Chinese)

Isolation, Identification and Drug Resistance Analysis of a Bovine Buttiauxella agrestis

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