亚洲黑熊源阴沟肠杆菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2018.08.037

Abstract

Abstract:

To determine the cause of the death of an Asian Black bear (Ursus thibetanus) at a park zoo in Guiyang,the death Black bear internal organs were collected for bacterial isolation and culture,cell morphology observation,physiological and biochemical identification,16S rRNA gene sequence analysis and mouse infection tests.The results showed that a round,convex,smooth surface,well-defined,medium-sized milky white colony was isolated from the dead Black bear lung specimen as gram-negative bacillus by microscopy.It could utilize the glucose,sucrose,mannitol,sorbitol,and citrate,non-lactose fermentation,non-generated sulfuretted hydrogen,positive arginine decarboxylation test and negative lysine decarboxylase,consistent with the biochemical characteristics of Enterobacter cloacae.1 419 bp fragments were generated by 16S rRNA primer amplification,and the similarity reached to 99.9% with the sequences of Enterobacter cloacae by BLAST comparison with the sequences on NCBI.Mice were inoculated intraperitoneally with the isolated bacteria,and the mice were lethal.The median lethal dose (LD₅₀) was 7.94×10⁸ CFU/mL.Drug susceptibility testing found that the isolates maintained high sensitivity to commonly used antimicrobial agents such as amikacin,ofloxacin,norfloxacin and ciprofloxacin.The results of this test showed that Enterobacter cloacae caused the death of the Asian Black bear.

Key words: Ursus thibetanus; Enterobacter cloacae; isolation; identification

CLC Number:

S855.1

SU Haijun, WANG Yeying, HE Xinwei, WAN Run, WEN Ming. Isolation, Identification and Drug Resistance Analysis of Enterobacter cloacae from Asian Black Bear (Ursus thibetanus)[J]. , 2018, 45(8): 2341-2346.

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Isolation, Identification and Drug Resistance Analysis of Enterobacter cloacae from Asian Black Bear (Ursus thibetanus)

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