复合植物精油对脂多糖诱导的断奶仔猪免疫应激的影响

doi:10.16431/j.cnki.1671-7236.2018.08.019

Abstract

Abstract:

The purpose of this research was to study the effect of OCT on the immune stress of weaned piglets induced by LPS.A total of 24 weaned piglets (Duroc×Large white×Landrace) were randomly assigned to three treatments at 28 days:Control group (based diet,injection of saline),LPS group (basic diet,injection of LPS) and OCT group (basal diet+50 mg/kg OCT,injection of LPS).Each treatment included 8 piglets that were subdivided equally to 8 pens,each pen had 1 piglets.On 21th day of the experiment,piglets in the LPS and OCT groups were injected intraperitoneally with LPS (100 μg/(kg·BW)),whereas piglets in the control group were injected intraperitoneally with the same volume of physiological saline.Blood samples were obtained 3 h after injection for analysis of white blood cell differential counts.6 h after LPS challenged,the pigs were slaughtered and thymus and spleen were taken for measuring the related genes IL-4,IL-6,IL-10,TNF-α,NF-κB,and STAT3 mRNA.The result showed that:①Compared with control group,LPS stimulation could lead to a significant reduction in the number of the eosinophils,neutrophils,lymphocytes,monocytes,white blood cells in the blood (P<0.05).Compared with LPS group,adding OCT could significantly increase blood levels of white blood cells (P<0.05).②Compared with control group,after injection of LPS,mRNA levels of IL-6 and STAT3 genes significantly increased in spleen and thymus (P<0.05);mRNA levels of IL-4,TNF-α in spleen and IL-4,TNF-α,NF-κB genes mRNA levels in thymus had been significantly reducted (P<0.05).Compared with LPS group,adding OCT could significantly reduce IL-6 and STAT3 mRNA levels in both spleen and thymus (P<0.05),and significantly increase IL-4,IL-10,TNF-α and NF-κB genes mRNA levels in thymus (P<0.05).In conclusion,LPS could induce immune stress in weaned pigs,whereas OCT could improve its immunity and alleviate inflammation to some extent.

Key words: compound plant of essential oil (OCT); piglets; lipopolysaccharide(LPS); immune stressed

CLC Number:

S816.75

XU Haiwang, YU Kui, WU Mengjun, LIU Wenkai, LI Peng, YI Dan, WANG Lei, HOU Yongqing. The Effect of Compound Plant Essential Oil on LPS-induced Immune-stressed Weaned Pigs[J]. , 2018, 45(8): 2197-2203.

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The Effect of Compound Plant Essential Oil on LPS-induced Immune-stressed Weaned Pigs

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