藏草乌水煎提取液体外杀灭羊虱蝇药效及皮肤毒性试验研究

doi:10.16431/j.cnki.1671-7236.2018.05.036

Abstract

Abstract:

The study was aimed to determine the efficacy and toxicity of Aconitum extract on Melophagus ovinus and it's skin toxicity,irritation and allergy in vitro,in order to provide a theoretical basis for the development,utilization and clinical application of Tibetan Aconitum.1%,2%,4%,8% and 10% Tibetan Aconitum extract were dropped in plates lined with absorbent paper and Melophagus ovinus to observe the killing efficacy on Melophagus ovinus at 1,2,4,8 h,respectively.The same dose of 1%,2% Tibetan Aconitum extract was taken to apply evenly in rabbits intact and damaged skin to detect the skin toxicity,irritation and allergy of Tibetan Aconitum extract.The results showed that at 1 h after administration,all drug groups and positive control group showed a kill effect on Melophagus ovinus in the different levels,and at 4 h after administration,the killing rate of positive control group was 100%,while that was 70% of 1% and 2% Tibetan Aconitum extract groups at 8 h with 2% drug dose showed the better effect.The skin toxicity test showed that rabbits skin had no erythema and edema,the body weight was normal,and the skin irritation test showed that the extract had no irritation to rabbits skin,while the results of skin allergy showed the it had no allergy to rabbits.In conclusion,Tibetan Aconitum extract had the good killing effects on Melophagus ovinus in vitro,and the skin medication was safe.

Key words: Aconitum extract; Melophagus ovinus; pharmacodynamics; irritation; toxicity; allergy

CLC Number:

S859.79⁺5

SONG Xiangdong, HU Yonghao, BAN Dan, LIANG Jianping, HU Yuyao, WUJIN Cuomu, SELANG Yuzhen, YUAN Zhenjie, GESANG Zhuoga, LABA Cidan, HAO Baocheng. Study on the Efficacy and Toxicity of Aconitum Extract on Melophagus ovinus in vitro[J]. , 2018, 45(5): 1408-1416.

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Study on the Efficacy and Toxicity of Aconitum Extract on Melophagus ovinus in vitro

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