杜寒绵羊多胎性能候选基因BMPR-1B和BMP15的研究

doi:10.16431/j.cnki.1671-7236.2018.05.024

Abstract

Abstract:

In order to investigate the mechanism of prolificacy in Duhan sheep at molecular level,the ear tissues of 87 ewes were collected from multiparous Duhan ewes in Shanxi province.The BMP15 and BMPR-1B genes were selected as candidate genes,and its relationship with prolificacy,which was associated with the litter size and the primary weight of lambs,was analyzed by the methods of PCR-SSCP and PCR-RFLP.The results showed that A→G mutation occurred in Duhan sheep at 746 bp of the coding region of BMPR-1B gene, at the same time three genotypes were detected:AA,AG and GG,the genotype frequency of AG was 0.5172, higher than that of GG (0.2184) and AA (0.2644),the allele frequency of A (0.5230) was slightly higher than G(0.4770) with A and AG was the dominance allele and genotype,respectively.And the site was in Hardy-Weinberg equlilbrium in Duhan sheep.There was no mutation occurred at 864 bp and also no mutation occurred at V31D and S300G sites in BMP15 gene.The number of lambs of GG and AG genotypes were extremely significantly higher than AA genotype (P<0.01) in this population,but there was no distinct difference between three genotypes in the primary weight of lamb(P>0.05).It was concluded that the BMPR-1B gene was a major gene affected reproductive traits in Duhan sheep, which could be used as a molecular marker to perform molecular assisted selection (MAS) in Duhan sheep.The influence of BMP15 gene mutation affecting prolific ability could be preliminarily excluded in Duhan sheep.

Key words: Duhan sheep; fecundity trait; BMPR-1B gene; BMP15 gene

CLC Number:

S813

GUO Huihui, LI Jun, LUO Huidi, NIU Jinguo. Study of BMPR-1B and BMP15 as Candidate Genes for Prolificacy of Duhan Sheep[J]. , 2018, 45(5): 1312-1318.

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Study of BMPR-1B and BMP15 as Candidate Genes for Prolificacy of Duhan Sheep

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