广西畜禽产品中沙门氏菌血清型、耐药性及耐药基因调查

doi:10.16431/j.cnki.1671-7236.2018.03.027

Abstract

Abstract:

To investigate the serotype distribution and drug resistance with the prevalence of resistance genes (bla_TEM,bla_CTX-M,oqxA,oqxB,qnrA and aac(6')-Ⅰb-cr) of Salmonella isolated from livestock and poultry products in Guangxi,Salmonella and its serotype were detected.Antibiotic resistance of 80 isolates to 24 kinds of antibiotics were determined by antibiotic susceptibility test according to the K-B method.PCR was used to detected the drug resistance genes.The results showed that 176 strains of Salmonella belonged to 26 serotypes of 5 serogroups.The main dominant serogroups were B,E and C groups with the prevalence rates of 60.23%(106/176),18.75%(33/176) and 15.91%(28/176),respectively.The main dominant serotypes were S.Derby,S.Typhimurium and S.London with the prevalence rates of 35.23%(62/176),11.93%(21/176) and 9.66%(17/176),respectively.The 80 isolated strains showed different resistance levels to the 24 kinds of antibiotics.The resistance rates to sulfamethoxazole,lincomycin and rifamycin were higher than 90.00% followed by the resistance rates to penicillin,ampicillin,amoxicillin,doxycycline,cefradine,cefalexin,ceftriaxone,ceftazidime,cefotaxime and azithromycin were between 50.00% to 90.00%,the resistance rates to ciprofloxacin,ofloxacin and florfenicol were lower than 10.00%.All of them were multiple drug resistance as they resisted 2 kinds of antibiotics in minimum and 17 kinds of antibiotics in maximum and majority of them resisted 10 to 16 kinds of antibiotics which accounted for 78.75%(63/80).PCR results showed that the prevalence rates of bla_TEM,bla_CTX-M,oqxA,oqxB,qnrA and aac(6')-Ⅰb-cr genes were 98.75%(79/80),26.25%(21/80),26.25%(21/80),21.25%(17/80),16.25%(13/80) and 50.00%(40/80),respectively.The results indicated that in Guangxi,serotypes of Salmonella in animal products were distributed in a variety of ways.The drug resistance of the isolated strains were very serious,and there was a great relationship between the drug resistance and the prevalence of drug resistance genes.

Key words: Salmonella; serotype; drug resistance; drug resistance gene; livestock and poultry products

CLC Number:

S852.61

ZHONG Shuhong, FENG Shiwen, LI Jun, ZHOU Qingan, CHEN Zexiang, XU Lishi, LIU Feng, MA Chunxia, HU Shuai, PAN Yan. Investigation on Serotype, Drug Resistance and Drug Resistance Gene of Salmonella in Livestock and Poultry Products of Guangxi[J]. , 2018, 45(3): 770-780.

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Investigation on Serotype, Drug Resistance and Drug Resistance Gene of Salmonella in Livestock and Poultry Products of Guangxi

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