反刍动物饲用抗生素替代物研究进展

doi:10.16431/j.cnki.1671-7236.2018.01.010

Abstract

Abstract:

Misuse of antibiotics imperils the development of animal husbandry in China, it is important to find reliable alternatives to antibiotics to reduce the use of antibiotics. This review summarizes the most common antibiotic feed additives already being used in ruminant feed, and gives an overview of the mechanism of alternative feed additives, such as probiotics, plant extracts, exogenous enzymes, organic acid, antibiotic peptides and oligosaccharides. The influences of alternative feed additives on rumen fermentation, immunity, microbial flora and production performance are presented. Their utility and limitations in modern ruminant production are also discussed. It can provide basis and references for further research and application of alternatives to antibiotics.

Key words: ruminant; antibiotic; alternatives to antibiotic; mechanism

CLC Number:

S816.7

JIN Chunjia, YE Gengping, TANG Xinren. Research Advances on Alternatives to Antibiotic Feed Additives in Ruminant Production[J]. , 2018, 45(1): 77-85.

References

[1] 王加启. 现代奶牛养殖科学[M]. 北京,中国农业出版社, 2006. WANG J Q. Modern Dairy Breeding Science[M]. Beijing:China Agriculture Press, 2006. (in Chinese)
[2] 杨波. 饲用抗生素的使用现状及面临的挑战[J]. 饲料工业,2007,22:3-5. YANG B. Present situation and challenges of feed antibiotics[J]. Feed Industry, 2007,22:3-5. (in Chinese)
[3] SEAL B S, LILLEHOJ H S, DONOVAN D M, et al. Alternatives to antibiotics:A symposium on the challenges and solutions for animal production[J]. Animal Health Research Reviews, 2013, 14(1):78-87.
[4] 代美纯子,戴春桃. 农村养殖业抗生素使用现状与应对措施——以张家界市为例[J]. 现代农业科技,2016,18:246-250. DAIMEI C Z, DAI C T. Present situation and countermeasures of antibiotics in rural animal breeding——Taking Zhangjiajie as an example[J]. Modern Agricultural Science and Technology, 2016,18:246-250. (in Chinese)
[5] 周庆民, 薄俊平, 冯万宇, 等. 抗生素在奶牛临床上的使用现状[J]. 中国乳业, 2009, 6:56-59. ZHOU Q M, BO J P, FENG W Y, et al. The current situation of antibiotic in dairy production[J].China Dairy, 2009,6:56-59. (in Chinese)
[6] DUPONT H L. The growing threat of foodborne bacterial enteropathogens of animal origin[J]. Clinical Infectious Diseases, 2007, 45(10):1353-1361.
[7] CASTANON J I R. History of the use of antibiotic as growth promoters in European poultry feeds[J]. Poultry Science, 2007, 86(11):2466-2471.
[8] 农业部.《饲料药物添加剂使用规范》[J]. 饲料与畜牧,2001,4:5-15. Ministry of Agriculture. Guidelines on the use of drug additives in feed[J]. Feed and Animal Husbandry, 2001,4:5-15. (in Chinese)
[9] JOUANY J P, MORGAVI D P. Use of ‘natural’ products as alternatives to antibiotic feed additives in ruminant production[J]. Animal, 2007, 1(10):1443-1466.
[10] WAGHORN G C, TAVENDALE M H, WOODFIELD D R. Methanogenesis from forages fed to sheep[A].Proceedings of the New Zealand Grassland Association[C]. 2002.
[11] MOLAN A L, WAGHORN G C, MIN B R, et al. The effect of condensed tannins from seven herbages on Trichostrongylus colubriformis larval migration in vitro[J]. Folia Parasitologica, 2000, 47(1):39-44.
[12] MCINTOSH F M, WILLIAMS P, LOSA R, et al. Effects of essential oils on ruminal microorganisms and their protein metabolism[J]. Applied and Environmental Microbiology, 2003, 69(8):5011-5014.
[13] BROGDEN K A, ACKERMANN M, MCCRAY P B, et al. Antimicrobial peptides in animals and their role in host defences[J]. International Journal of Antimicrobial Agents, 2003, 22(5):465-478.
[14] WANG Y, MCALLISTER T A. Rumen microbes, enzymes and feed digestion——A review[J]. Asian Australasian Journal of Animal Sciences, 2002, 15(11):1659-1676.
[15] YANG W Z, BEAUCHEMIN K A, RODE L M. Effects of an enzyme feed additive on extent of digestion and milk production of lactating dairy cows[J]. Journal of Dairy Science, 1999, 82(2):391-403.
[16] 徐晓锋, 金亚东. 寡糖对奶牛瘤胃发酵及乳脂调控的研究进展[J]. 畜牧与兽医, 2016,48(1):116-119. XU X F, JIN Y D. Study progress of oligosaccharides on ruminal fermentation and milk regulation in dairy cows[J]. Animal Husbandry & Veterinary Medicine, 2016, 48(1):116-119. (in Chinese)
[17] BOUDRA H. Mycotoxins:An insidiously menacing factor for the quality of forages and the performances of the ruminants[J]. Fourrages, 2009,199:265-280.
[18] QADIS A Q, YO-HAN K I M, IKUTA K, et al. Effects of a bacterial probiotic on ruminal pH and volatile fatty acids during subacute ruminal acidosis (SARA) in cattle[J]. Journal of Veterinary Medical Science, 2016, 78(10):1595-1600.
[19] PINLOCHE E, MCEWAN N, MARDEN J P, et al. The effects of a probiotic yeast on the bacterial diversity and population structure in the rumen of cattle[J]. PLoS One, 2013, 8(7):e67824.
[20] 王卫正,刘青,张香云,等. 酵母培养物对奶牛生产性能及抗氧化功能的影响[J].中国畜牧杂志,2016,19:61-66. WANG W Z, LIU Q, ZHANG X Y. Effects of yeast culture on production performance and antioxidant function in dairy cow[J]. Chinese Journal of Animal Science,2016,19:61-66. (in Chinese)
[21] 周盟. 植物乳杆菌和枯草芽孢杆菌及其复合菌在断奶仔猪和犊牛日粮中的应用研究[D].乌鲁木齐:新疆农业大学, 2013. ZHOU M. Study on application of Lactobacillus Plantar, Bacillus subtilis and complex-probiotics in diets for weaned piglets and calves[D]. Urumqi:Xinjiang Agricultural University, 2013. (in Chinese)
[22] ZHANG R, ZHOU M, TU Y, et al. Effect of oral administration of probiotics on growth performance, apparent nutrient digestibility and stress-related indicators in Holstein calves[J].Journal of Animal Physiology and Animal Nutrition, 2016, 100(1):33-38.
[23] HE Z X, FERLISI B, ECKERT E, et al. Supplementing a yeast probiotic to pre-weaning Holstein calves:Feed intake, growth and fecal biomarkers of gut health[J]. Animal Feed Science and Technology, 2017, 226:81-87.
[24] ALUGONGO G M, XIAO J X, CHUNG Y H, et al. Effects of Saccharomyces cerevisiae fermentation products on dairy calves:Performance and health[J]. Journal of Dairy Science, 2017, 100(2):1189-1199.
[25] CHAUCHEYRAS-DURAND F, MASSéGLIA S, FONTY G, et al. Influence of the composition of the cellulolytic flora on the development of hydrogenotrophic microorganisms, hydrogen utilization, and methane production in the rumens of gnotobiotically reared lambs[J]. Applied and Environmental Microbiology, 2010, 76(24):7931-7937.
[26] 李元恒, 金龙, 韩国栋, 等. 植物单宁在反刍动物营养和健康养殖作用中的研究进展[J]. 草地学报, 2013, 21(6):1043-1051. LI Y H, JIN L, HAN G D, et al. Effects of plant tannin on the nutrition and healthy production of ruminant:A review[J]. Acta Agrectir Sinica, 2013, 21(6):1043-1051. (in Chinese)
[27] MIN B R, BARRY T N, ATTWOOD G T, et al. The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages:A review[J]. Animal Feed Science and Technology, 2003, 106(1):3-19.
[28] MIN B R, PINCHAK W E, FULFORD J D, et al. Wheat pasture bloat dynamics, in vitro ruminal gas production, and potential bloat mitigation with condensed tannins[J]. Journal of Animal Science, 2005, 83(6):1322-1331.
[29] 徐晓锋. 缩合单宁与水解单宁对奶牛日粮氮利用影响及作用机制的研究[D]. 北京:中国农业大学,2013. XU X F. Study on mechanism of nitrogen utilization in diet of dairy cows with condensed and hydrolyzable tannins addition[D]. Beijing:China Agricultural University, 2013. (in Chinese)
[30] TANNER G J, MOATE P J, DAVIS L H, et al. Proanthocyanidins (condensed tannin) destabilise plant protein foams in a dose dependent manner[J]. Crop and Pasture Science, 1995, 46(6):1101-1109.
[31] 刘立成,刘娣,邹红菲,等. 单宁酸对奶牛生产性能及甲烷排放的影响[J]. 饲料工业,2014,17:108-111. LIU L C, LIU T, ZOU H F, et al. The Effect of tannins on the dairy cow performance and the methane emission[J]. Feed Industry, 2014,17:108-111. (in Chinese)
[32] AUFRERE J, PONCET C, GAYRAUD P, et al. Nutritional interest of growing mixtures of grasses and legumes with and without tannins[A].Sustainable Grassland Productivity[C]. Badajoz, Spain:Proceedings of the 21st General Meeting of the European Grassland Federation, 2006.
[33] TRAORE F, FAURE R, OLLIVIER E, et al. Structure and antiprotozoal activity of triterpenoid saponins from Glinus oppositifolius[J]. Planta Medica, 2000, 66(4):368-371.
[34] SANTOSO B, MWENYA B, SAR C, et al. Effects of supplementing galacto-oligosaccharides, Yucca schidigera or nisin on rumen methanogenesis, nitrogen and energy metabolism in sheep[J]. Livestock Production Science, 2004, 91(3):209-217.
[35] 冯志华. 蒺藜皂苷对奶牛瘤胃发酵、甲烷产量、抗氧化功能及免疫功能的影响[D]. 保定:河北农业大学, 2013. FENG Z H. Effects of gross saponin of Tribulus terrestris on rumen fermentation, methane production, antioxidant capacity and immune function in dairy cows[D].Baoding:Hebei Agricultural University, 2013. (in Chinese)
[36] JOHNSON I T, GEE J M, PRICE K, et al. Influence of saponins on gut permeability and active nutrient transport in vitro[J]. The Journal of Nutrition, 1986, 116(11):2270-2277.
[37] SANTOS M B, ROBINSON P H, WILLIAMS P, et al. Effects of addition of an essential oil complex to the diet of lactating dairy cows on whole tract digestion of nutrients and productive performance[J]. Animal Feed Science and Technology, 2010, 157(1):64-71.
[38] CASTILLEJOS L, CALSAMIGLIA S, FERRET A, et al. Effects of dose and adaptation time of a specific blend of essential oil compounds on rumen fermentation[J]. Animal Feed Science and Technology, 2007, 132(3):186-201.
[39] TAGER L R, KRAUSE K M. Effects of essential oils on rumen fermentation, milk production, and feeding behavior in lactating dairy cows[J]. Journal of Dairy Science, 2011, 94(5):2455-2464.
[40] 姚喜喜, 吴建平, 刘婷, 等. 全混合日粮中添加牛至精油对泌乳期荷斯坦奶牛生产性能和蹄病发生率的影响[J]. 草业科学, 2016, 33(2):299-304. YAO X X, WU J P, LIU T, et al. Effects of adding the oregano essential oil to the total mixed ration on production performance[J]. Pratacultural Science, 2016, 33(2):299-304. (in Chinese)
[41] 陈昊, 刘婷, 姚喜喜, 等. 牛至精油对荷斯坦奶牛乳房炎和腹泻发病率的影响[J]. 中国草食动物科学, 2015, 2:39-41. CHEN H, LIU T, YAO X X, et al. Effect of oregano essential oil on the mastitis and diarrhea of Holstein cows[J]. China Herbivore Science, 2015, 2:39-41. (in Chinese)
[42] PATRA A K. Enteric methane mitigation technologies for ruminant livestock:A synthesis of current research and future directions[J]. Environmental Monitoring and Assessment, 2012, 184(4):1929-1952.
[43] KOLVER E S, ASPIN P W, JARVIS G N, et al. Fumarate reduces methane production from pasture fermented in continuous culture[A].Conference of the New Zealand Society of Animal Production, 2004.
[44] YANG C J, MAO S Y, LONG L M, et al. Effect of disodium fumarate on microbial abundance, ruminal fermentation and methane emission in goats under different forage:Concentrate ratios[J]. Animal, 2012, 6(11):1788-1794.
[45] 刘南南. 日粮碳水化合物平衡指数和延胡索酸对山羊瘤胃发酵、微生物区系和甲烷产生的影响[D]. 杨凌:西北农林科技大学, 2014. LIU N N. Effects of dietary physical effective fiber to rumen degradable starch ratios and fumaric acids on rumen fermentation, microbial communities and methane emission in dairy goats[D]. Ynagling:Northwest A&F University, 2014. (in Chinese)
[46] FOLEY P A, KENNY D A, CALLAN J J, et al. Effect of malic acid supplementation on feed intake, methane emission, and rumen fermentation in beef cattle[J]. Journal of Animal Science, 2009, 87(3):1048-1057.
[47] BEAUCHEMIN K A, MCGINN S M. Methane emissions from beef cattle:Effects of fumaric acid, essential oil, and canola oil[J]. Journal of Animal Science, 2006, 84(6):1489-1496.
[48] HINO T, TAKESHI K, KANDA M, et al. Effects of aibellin, a novel peptide antibiotic, on rumen fermentation in vitro.[J]. Journal of Dairy Science, 1993, 76(8):2213-2221.
[49] LEE S S, HSU J T, MANTOVANI H C, et al. The effect of bovicin HC5, a bacteriocin from Streptococcus bovis HC5, on ruminal methane production in vitro[J]. FEMS Microbiology Letters, 2002, 217(1):51-55.
[50] SAR C, MWENYA B, PEN B, et al. Effect of nisin on ruminal methane production and nitrate/nitrite reduction in vitro[J]. Crop and Pasture Science, 2005, 56(8):803-810.
[51] 王佩, 范燕茹, 金鑫, 等. 枯草芽孢杆菌对绵羊瘤胃上皮细胞β-防御素-1表达的影响[J]. 畜牧兽医学报, 2015,46(5):760-767. WANG P, FAN Y R, JIN X, et al. Effects of Bacillus subtilis on the expression of SBD-1 in cultured ruminal epithelial cells of sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2015,46(5):760-767. (in Chinese)
[52] 金鑫, 张曼, 范燕茹, 等. 酿酒酵母菌对绵羊瘤胃上皮细胞β-防御素-1(SBD-1)基因表达的影响[J]. 中国农业科学, 2015, 48(19):3910-3918. JIN X, ZHANG M, FAN Y R, et al. Effects of Saccharomyces cerevisiae on the expression of SBD-1 in cultured ruminal epithelial cells of sheep[J]. Scientia Agricultura Sinica, 2015, 48(19):3910-3918. (in Chinese)
[53] 范燕茹, 王佩, 金鑫, 等. 乳酸杆菌诱导绵羊瘤胃上皮细胞内SBD-1的表达[J]. 中国兽医学报, 2016, 36(4):604-612. FAN Y R, WANG P, JIN X, et al. The expression of SBD-1 in the rumen epithelium cells of sheep induced by Lactobacillus[J]. Chinese Journal of Veterinary Science, 2016, 36(4):604-612. (in Chinese)
[54] JALILVAND G, ODONGO N E, LÍPEZ S, et al. Effects of different levels of an enzyme mixture on in vitro gas production parameters of contrasting forages[J]. Animal Feed Science and Technology, 2008, 146(3):289-301.
[55] KNOWLTON K F, MCKINNEY J M, COBB C. Effect of a direct-fed fibrolytic enzyme formulation on nutrient intake, partitioning, and excretion in early and late lactation Holstein cows[J]. Journal of Dairy Science, 2002, 85(12):3328-3335.
[56] 车超. 纤维素酶、尿素对奶牛生产性能和生化指标的影响[D]. 武汉:华中农业大学, 2006. CHE C. Effects of cellulase and urea on the production performance and biochemical indexes of dairy cows[D]. Wuhan:Huazhong Agricultural University, 2006. (in Chinese)
[57] ROMERO J J, MACIAS E G, MA Z X, et al. Improving the performance of dairy cattle with a xylanase-rich exogenous enzyme preparation[J]. Journal of Dairy Science, 2016, 99(5):3486-3496.
[58] BILIK K. Effect of adding fibrolytic enzymes to dairy cow rations on digestive activity in the rumen[J]. Annals of Animal Science, 2010, 10(2):127-137.
[59] LU Q, WU J, WANG M, et al. Effects of dietary addition of cellulase and a Saccharomyces cerevisiae fermentation product on nutrient digestibility, rumen fermentation and enteric methane emissions in growing goats[J]. Archives of Animal Nutrition, 2016, 70(3):224-238.
[60] WANG L, XUE B. Effects of cellulase supplementation on nutrient digestibility, energy utilization and methane emission by Boer crossbred goats[J]. Asian-Australasian Journal of Animal Sciences, 2016, 29(2):204.
[61] 任海军. 壳聚糖对奶牛产奶性能和免疫功能影响的研究[D].呼和浩特:内蒙古农业大学, 2008. REN H J. Effects of chitosan on production of milk and immune function in dairy cows[D].Hohhot:Inner Mongolia Agricultural University, 2008. (in Chinese)
[62] 鲍延安, 邢淑芳, 徐庆龙. 甘露寡糖对荷斯坦奶牛产奶量及乳常规的影响[J]. 当代畜牧, 2008(9):57-60. BAO Y A, XING S F, XU Q L. Effects of mannan oligosaccharides on milk production and milk routine of Holstein dairy cows[J]. Contemporary Animal Husbandry, 2008(9):57-60. (in Chinese)
[63] CASTRO J J, GOMEZ A, WHITE B A, et al. Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves. 1. Effects of prebiotic supplementation depend on site and age[J]. Journal of Dairy Science, 2016, 99(12):9682-9702.
[64] HTUN A, SATO T, HANADA M. Effect of difructose anhydride Ⅲ supplementation on passive immunoglobulin G transfer and serum immunoglobulin G concentration in newborn Holstein calves fed pooled colostrum[J]. Journal of Dairy Science, 2016,99(7):5701-5706.
[65] FRANKLIN S T, NEWMAN M C, NEWMAN K E, et al. Immune parameters of dry cows fed mannan oligosaccharide and subsequent transfer of immunity to calves[J]. Journal of Dairy Science, 2005, 88(2):766-775.
[66] HEINRICHS A J, JONES C M, HEINRICHS B S. Effects of mannan oligosaccharide or antibiotics in neonatal diets on health and growth of dairy calves[J]. Journal of Dairy Science, 2003, 86(12):4064-4069.

Research Advances on Alternatives to Antibiotic Feed Additives in Ruminant Production

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