›› 2017, Vol. 44 ›› Issue (6): 1811-1817.doi: 10.16431/j.cnki.1671-7236.2017.06.033

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Research Progress on Diagnosis Technology of Dairy Cow Mastitis Pathogens

WANG Dan1, YANG Feng1, LI Xin-pu1, LUO Jin-yin1, LIU Long-hai1, ZHANG Zhe1, ZHANG Ya-ru1, ZHANG Li-li1,2, LI Hong-sheng1   

  1. 1. Key Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Discovery, Ministry of Agriculture, Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China;
    2. School of Public Health, Lanzhou University, Lanzhou 730000, China
  • Received:2016-12-05 Online:2017-06-20 Published:2017-06-28

Abstract:

Dairy cow mastitis is one of the most costly diseases affecting the development of the world dairy industry. It causes a serious economic loss to dairy farming, and affects the yield and quality of milk, endangers human health. The cause of dairy cow mastitis is complex, but the main cause is pathogen infection, particularly Staphylococcus, Streptococcus and intestinal bacterial infection. The rapid diagnosis of pathogenic bacteria of mastitis is crucial for the effectively treatment of the dairy cow mastitis. In order to provide theoretical basis for developing rapid, specific and sensitive of dairy cow mastitis pathogens diagnosis, the author summarized the microbiological and biochemical assay method in routine used of dairy cow mastitis pathogen diagnosis, automatic microbial identification system, resazurin trace plate method, immune diagnosis method, the nucleic acid probe hybridization, 16S rRNA gene sequences of sequencing, PCR, gene chip and LAMP etc, and analyzed the advantages and disadvantages of these methods.

Key words: dairy cow; mastitis; pathogenic bacteria; diagnosis technology

CLC Number: