猪源大肠杆菌O157∶H7毒力差异株的转录组测序分析

doi:10.16431/j.cnki.1671-7236.2017.06.032

Abstract

Abstract:

In order to gain an insight of the transcriptomes difference in E.coli O157:H7 strains of different virulence,enrich the E.coli O157:H7 transcriptome data,two E.coli O157:H7 strains of different virulence were selected,then the transcriptome libraries of the strains were constructed using Illumina HiSeq^TM 2000 sequencing technique.The bioinformatics methods were used subsequently,such as sequencing assess and gene function annotation. The results showed that 3 113 118 reads were obtained in the high virulence strain,and 2 944 912 reads were obtained in normal virulence strain.83.76% and 78.97% reads could be compared to the E.coli O157:H7 reference genome sequence,respectively. Comparison with the transcriptome of normal virulence strain,941 differentially expressed genes were found in the high virulence strain,including 637 up-regulated genes and 304 down-regulated genes.GO function annotation classifications indicated that the differentially expressed genes were divided into different function categories,in which many functional categories were mainly involved,such as catalytic activity,binding,transporter activity,receptor activity,enzyme regulator activity,localization,biological regulation,locomotion. KEGG analysis showed 425 genes were annotated to 160 metabolic pathways. Metabolic,ribosome,flagellar assembly,pyrimidine metabolism,starch and sucrose metabolism,bacterial chemotaxis pathways were significantly enriched. The transcriptome study of E.coli O157:H7 strains of different virulence using high-throughput sequencing technology revealed the number of differentially expressed genes in different virulence,obtained the function,classification and metabolic pathway of differentially expressed genes,which enriched the transcriptome information of E.coli O157:H7,and laid a foundation for the study on related genes with virulence traits and molecular mechanisms in E.coli O157:H7.

Key words: E.coli O157:H7; transcriptome; GO classification; KEGG pathway

CLC Number:

S852.61

PENG Hao, LI Jun, YANG Wei, CHEN Ze-xiang, PAN Yan, FENG Shi-wen, ZENG Yun. Transcriptome Sequencing Analysis of E.coli O157:H7 Strains of Different Virulence from Pigs[J]. , 2017, 44(6): 1804-1810.

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Transcriptome Sequencing Analysis of E.coli O157:H7 Strains of Different Virulence from Pigs

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