羊源D型多杀性巴氏杆菌感染小鼠脾脏转录组分析

doi:10.16431/j.cnki.1671-7236.2021.12.007

Abstract

Abstract: In order to illustrate the transcriptomic profile of spleen in mice upon Pasteurella multocida infection, the mouse model of P.multocida infection was established by intraperitoneal injection.Transcriptome sequencing technology was performed to obtain spleen transcriptome data of infected and normal mice.Furthermore, COG, KOG, eggNOG, GO, KEGG database were utilized to accomplish functional annotation and the differentially expressed genes (DEGs) analysis.In order to screen out the genes that played the key role in immune pathways, DEGs which were significantly enriched in several immune pathways, were used to perform protein interaction analysis with STRING software and KEGG mapper.Finally, 10 key genes were randomly selected for Real-time RT-PCR verification.Transcriptome analysis results showed that compared with the normal group, 3 380 DEGs were screened in the infection group (P<0.01, log₂|FoldChange|≥0.5), of which 1 691 DEGs were up-regulated and 1 689 DEGs were down-regulated.Gene function enrichment analysis showed that DEGs in spleen of the infected group mainly played the function of signal transduction.The cytokine-cytokine receptor interaction pathway, chemokine signal pathway, HIF-1 signal pathway and TNF signal pathway were significantly enriched in the context of P.multocida infection.Protein interaction analysis screened out 28 core DEGs.After verification with Real-time RT-PCR, the expression of 9 genes were consistent with sequencing results, namely C3, Cd4, Cxcl13, Lck, Gnai1, Grap2, IL-6, Cxcr6 and Serping1 genes.This study demonstrated that the spleen played the essential immune role in resisting the invasion of P.multocida and could provide valuable information on the molecular mechanism of the interaction between goat-derived P.multocida and the host.

Key words: Pasteurella multocida; transcriptome analysis; spleen; immune response

CLC Number:

S852.61

LI Bin, CHEN Qiaoling, CHENG Yiwen, CHEN Jie, ZHANG Zhenxing, AN Qi, HUANG Huixian, LIU Zhiyong, ZHAI Zhe, MAN Churiga, WANG Fengyang, DU Li, CHEN Si. Transcriptome Analysis of Spleen of Mouse Infected with Pasteurella multocida Type D from Goat[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4391-4402.

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Transcriptome Analysis of Spleen of Mouse Infected with Pasteurella multocida Type D from Goat

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