牛源多杀性巴氏杆菌血清分型及毒力相关基因的检测研究

doi:10.16431/j.cnki.1671-7236.2020.06.031

Abstract

Abstract: In order to determine the distribution of serotypes,ST types and virulence related genes of Pasteurella multocida (Pm) isolated from suspected cases in Northern Xinjiang,17 strains of Pm isolated from cattle were studied in this study.The methods of capsule multiplex PCR,LPS multiplex PCR,multilocus sequence typing (MLST) and PCR were used to detect the capsule type,lipopolysaccharide type,MLST type and the distribution of 7 classes of 25 virulence related genes in 17 strains of Pm isolates.The results showed that 13 strains of Pm were A:L3,ST was ST1,4 strains of Pm were B:L2,ST was ST44.The detection rate of 17 Pm virulence related genes (exbB,exbD,fimA,fur,hgbA,hsf2,nanB,oma87,ompA,ompH,plpB,psl,ptfA,sodA,sodC,tonB and tbpA) was 100%,and the detection rate of toxA gene was 0.The results showed that the main serotype of Pm,which isolated from parts of northern Xinjiang large-scale cattle farms, was A:L3:ST1.

Key words: Pasteurella multocida (Pm); capsule; lipopolysaccharide; multilocus sequence typing (MLST); virulence-associated gene

CLC Number:

S852.61

WU Qin, ZHANG Xingxing, GU Xiaoxiao, TAOQIAO Xiaoci, HUANG Xin, HAN Mengli, WU Tongzhong, ZHOU Xia, ZHONG Fagang. Study on Serotyping and Detection of Virulence-associated Genes of Pasteurella multocida Isolated from Bovine[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1910-1920.

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Study on Serotyping and Detection of Virulence-associated Genes of Pasteurella multocida Isolated from Bovine

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