金荞麦对脂多糖诱导小鼠小肠炎症的保护作用

doi:10.16431/j.cnki.1671-7236.2020.02.032

Abstract

Abstract: To explore the protective effect of Fagopyrum dibotrys on lipopolysaccharide (LPS)-induced inflammation model of the small intestine in mice,ICR mice were selected in the experiment.Different concentration of Fagopyrum dibotrys and the positive control- Rutin were added in drinking water for 14 days treatment,then intraperitoneal injection of LPS (10 mg/kg) was used to construct the model of acute enteritis.Finally,the results were evaluated by behavior changes,routine blood tests,histological sections and gene expressions of inflammation-related genes.The results showed that the intraperitoneal injection of 10 mg/kg LPS could successfully induced acute enteritis in mice.The addition of Fagopyrum dibotrys could reduce the weight loss of LPS-induced acute enteritis in a dose-dependent manner.Blood routine test showed that intraperitoneal injection of LPS caused a significant decrease in white blood count (WBC),red blood count (RBC),hemoglobin (HGB) and platelet count (PLT) in mice,and the addition of Fagopyrum dibotrys or Rutin could alleviate the blood routine abnormality caused by LPS injection to a certain extent.The hematoxylin-eosin (HE) staining showed that after intraperitoneal injection of LPS,the focal inflammatory cells were found infiltrated into the lamina propria with a large number,as well as the glandular epithelium with a small number.Also,the disorganized intestinal cells,nuclear division and nuclear fragmentation were observed.Furthermore,severely mucosal hyperemia and hemorrhage were observed.To a certain extent,the infiltration of inflammatory cells and mucosa bleeding could be suppressed by adding Fagopyrum dibotrys or Rutin in mice.RT-PCR results showed that intraperitoneal injection of LPS could significantly promote the mRNA expression level of inflammatory factors (i.e.IL-6,TNF-α and IL-1β),and the expression of TNF-α and IL-1β decreased by adding Fagopyrum dibotrys in a dose-dependent manner.The above results showed that Fagopyrum dibotrys or Rutin could effectively alleviate the inflammation of mice small intestine induced by LPS,the 12% adding of Fagopyrum dibotrys could achieve the effect of 100 mg/kg Rutin in mice somehow.

Key words: Fagopyrum dibotrys; lipopolysaccharide; enteritis; protective effect

CLC Number:

S816.5⁺1

TAN Ya, GAN Mailin, FAN Yuan, WANG Jing, ZHANG Xiong, HUANG Bo, ZHU Li, SHANG Yishun, SHI Kaizhi. A Protective Effects of Fagopyrum dibotrys on Lipopolysaccharide-induced Intestinal Inflammation in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 597-604.

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A Protective Effects of Fagopyrum dibotrys on Lipopolysaccharide-induced Intestinal Inflammation in Mice

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