基于网络药理学探究乌梅散加减方治疗猪传染性胃肠炎的作用机制

doi:10.16431/j.cnki.1671-7236.2022.11.036

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the mechanism of modified Wumei powder in the treatment of porcine transmissible gastroenteritis (TGE) by network pharmacology.【Method】 The active components and action targets of modified Wumei powder were obtained using Traditional Chinese Medicine System Pharmacology Database and Analysis Platform (TCMSP) and Swiss TargetPrediction, and the related targets of TGE were obtained using the Public Comparative Toxicology Genomics Database (CTD).The protein interaction (PPI) network diagram of the intersection targets of modified Wumei powder and TGE was drawn using STRING database.The GO function and KEGG pathway enrichment analysis of the intersection targets were performed by DAVID, so as to explore the main action mechanism of modified Wumei powder in the treatment of TGE.【Result】 A total of 125 active components such as quercetin, kaempferol and formononetin and 58 action targets such as JUN, TNF and STAT3 were screened from modified Wumei powder.GO functional enrichment analysis showed that a total of 138 items of biological process were obtained, including immunoreaction, generation of vascular endothelial factors, and positive regulation of pri-miRNA transcription by the RNA polymerase Ⅱ promoter.Cellular component was 13 items, involving extracellular region, extracellular space, membrane raft, etc.Molecular function was 32 items, involving cytokine activity, growth factor activity, and interleukin 2 (IL2) receptor activity.Enrichment analysis of KEGG pathway revealed that a total of 135 signaling pathways were identified, including IL17 signaling pathway, Th17 cell differentiation, TNF signaling pathway, and HIF-1 signaling pathway, which were the main pathways of modified Wumei powder in the treatment of TGE.【Conclusion】 The results above indicated that modified Wumei powder mainly regulated JUN, TNF and STAT3 targets by multiple active components such as quercetin, kaempferol and formononetin, and treated TGE by participating in IL17 signaling pathway and Th17 cell differentiation.

Key words: modified Wumei powder; pigs; transmissible gastroenteritis (TGE); network pharmacology

CLC Number:

S853.74

FAN Junyang, WU Wenyi, ZHANG Yuntian, JIN Yue, YANG Mingfan, ZHANG Hongying. Exploration on the Mechanism of Modified Wumei Powder in the Treatment of Porcine Transmissible Gastroenteritis Based on Network Pharmacology[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4447-4456.

References

[1] 李嘉琛, 吴华伟, 陈晓春, 等.猪传染性胃肠炎病毒的分离与鉴定[J].中国兽药杂志, 2018, 52(3):25-30. LI J C, WU H W, CHEN X C, et al.Isolation and identification of porcine Transmissible gastroenteritis virus[J].Chinese Journal of Veterinary Drug, 2018, 52(3):25-30.(in Chinese)
[2] 洪尼宁, 张登祥, 李涛, 等.贵州省猪传染性胃肠炎及呼吸冠状病毒病血清学调查[J].中国畜牧兽医, 2010, 37(12):180-182. HONG N N, ZHANG D X, LI T, et al.Serological survey for TGEV and PRCV antibodies among swine from Guizhou province[J].China Animal Husbandry & Veterinary Medicine, 2010, 37(12):180-182.(in Chinese)
[3] MA Q, PAN W, LI R, et al.Liu Shen capsule shows antiviral and anti-inflammatory abilities against novel coronavirus SARS-CoV-2 via suppression of NF-κB signaling pathway[J].Pharmacological Research, 2020, 158:104850.
[4] 张登鹤, 马正文.乌梅散加味治疗仔猪红黄白痢[J].中兽医学杂志, 2017, 1:28. ZHANG D H, MA Z W.Treatment of red, yellow and white dysentery of piglets with modified Wumei powder[J].Chinese Journal of Traditional Veterinary Science, 2017, 1:28.(in Chinese)
[5] 刘艳艳, 李明珠, 张善婷, 等.乌梅散加味辅助治疗犬球虫病[J].黑龙江畜牧兽医, 2012, 20:102-103. LIU Y Y, LI M Z, ZHANG S T, et al.Adjuvant treatment of coccidiosis in dog with modified Wumei powder[J].Heilongjiang Animal Science and Veterinary Medicine, 2012, 20:102-103.(in Chinese)
[6] 吴卫东, 陈松林.乌梅散加减方对猪传染性胃肠炎的治疗效果[J].畜牧兽医科技信息, 2018, 12:46-47. WU W D, CHEN S L.Therapeutic effect of modified Wumei powder recipe on transmissible gastroenteritis of swine[J].Chinese Journal of Animal Husbandry and Veterinary Medicine, 2018, 12:46-47.(in Chinese)
[7] 许家玉.乌梅散加减方治疗猪传染性胃肠炎效果研究[J].安徽农学通报, 2016, 22(8):107-110. XU J Y.Effect of porcine transmissible gastroenteritis treatment of modified Wumei powder[J].Anhui Agricultural Science Bulletin, 2016, 22(8):107-110.(in Chinese)
[8] 张慧茹, 张婷婷, 许枬, 等.基于NMR指导的六神曲成分研究[J].中草药, 2019, 50(16):3764-3768. ZHANG H R, ZHANG T T, XU Z, et al.NMR-based study of chemical constituents of Massa Medicata Fermentata[J].Chinese Traditional and Herbal Drugs, 2019, 50(16):3764-3768.(in Chinese)
[9] 唐旭, 赵成鹏, 段永福, 等.槲皮素通过调节SIRT1/NLRP3信号通路对实验性巨结肠大鼠肠损伤的影响[J].现代药物与临床, 2022, 37(4):673-680. TANG X, ZHAO C P, DUAN Y F, et al Effects of quercetin on intestinal injury in experimental megacolon rats by regulating SIRT1/NLRP3 signaling pathway[J]. Drugs & Clinic, 2022, 37(4):673-680.(in Chinese)
[10] 李雅娟, 孙姣姣, 刘广益, 等.山柰酚对脂多糖诱导小胶质细胞炎性介质释放与吞噬的影响[J].神经解剖学杂志, 2021, 37(2):182-188. LI Y J, SUN J J, LIU G Y, et al Inhibitory effect of kaempferol on lipopolysaccharide-induced inflammatory mediator release and phagocytosis of microglia[J].Chinese Journal of Neuroanatomy, 2021, 37(2):182-188.(in Chinese)
[11] 毛甜甜, 白衡, 贾宁.芒柄花素对免疫抑制小鼠小肠黏膜免疫功能的影响[J].动物营养学报, 2022, 34(4):2712-2720. MAO T T, BAI H, JIA N.Effects of formonetin on mucosa immunity function in small intestinal of immunosuppressive mice[J].Chinese Journal of Animal Nutrition, 2022, 34(4):2712-2720.(in Chinese)
[12] 罗钧秋, 杜建, 王岚, 等.猪传染性胃肠炎病毒感染对仔猪肠道屏障功能的影响及营养调控[J].南京农业大学学报, 2019, 42(5):787-792. LUO J Q, DU J, WANG L, et al.Effects of porcine Transmissible gastroenteritis virus infection on intestinal barrier function and nutrition strategy[J].Journal of Nanjing Agricultural University, 2019, 42(5):787-792.(in Chinese)
[13] LU W, WANG Y, ZHANG Q, et al.TNF-derived peptides inhibit tumour growth and metastasis through cytolytic effects on tumour lymphatics[J].Clinical and Experimental Immunology, 2019, 198(2):198-211.
[14] ZHU L, YANG X, MOU C, et al.Transmissible gastroenteritis virus does not suppress IFN-β induction but is sensitive to IFN in IPEC-J2 cells[J].Veterinary Microbiology, 2017, 199:128-134.
[15] 白欣宇, 张峰睿, 缪应雷.TNF-α拮抗剂与炎症性肠病合并肝炎病毒感染的关系[J].国际消化病杂志, 2021, 41(3):159-163. BAI X Y, ZHANG F R, MIAO Y L.Relationship between TNF-α antagonist and inflammatory bowel disease combined with Hepatitis virus infection[J].International Journal of Digestive Diseases, 2021, 41(3):159-163.(in Chinese)
[16] 方翔, 王民, 陈涛, 等.S1P-STAT3信号通路在炎症性疾病中的作用及其机制研究进展[J].生物学通报, 2021, 56(8):1-6. FANG X, WANG M, CHEN T, et al.Research progress on the role and mechanism of S1P-STAT3 signaling pathway in inflammatory disease[J].Bulletin of Biology, 2021, 56(8):1-6.(in Chinese)
[17] 董朝军, 刘宣毅, 李冕, 等.JAK2/STAT3信号通路在膝骨关节炎大鼠滑膜组织中的表达及其对SCGF-β及VEGF的调控作用[J].广西医科大学学报, 2021, 38(9):1735-1740. DONG C J, LIU Y Y, LI M, et al Expression of JAK2/STAT3 signaling pathway in synovium of knee osteoarthritis rats and its regulation on SCGF-β and VEGF[J].Journal of Guangxi Medical University, 2021, 38(9):1735-1740.(in Chinese)
[18] GAO J, BAI H, LI Q, et al.In vitro investigation of the mechanism underlying the effect of ginsenoside on the proliferation and differentiation of neural stem cells subjected to oxygen-glucose deprivation/reperfusion[J].International Journal of Molecular Medicine, 2018, 41(1):353-363.
[19] HORIGUCHI A, ASANO T, KURODA K, et al.STAT3 inhibitor WP1066 as a novel therapeutic agent for renal cell carcinoma[J].British Journal of Cancer, 2010, 102(11):1592-1599.
[20] 张伟, 涂敏芳, 邓焕华.转录因子ROR-γt与Th17介导的中性粒细胞性哮喘[J].中国免疫学杂志, 2018, 34(9):1366-1371. ZHANG W, TU M F, DENG H H.Transcription factor ROR-γt and neutrophilic asthma mediated by Th17[J].Chinese Journal of Immunology, 2018, 34(9):1366-1371.(in Chinese)
[21] WANG Y H, LIU Y J.The IL-17 cytokine family and their role in allergic inflammation[J].Current Opinion in Immunology, 2008, 20(6):697-702.
[22] 王岚.猪传染性胃肠炎病毒(TGEV)感染IPEC-J2细胞的炎症反应及亮氨酸的调控作用[D].雅安:四川农业大学, 2019. WANG L.The effect of leucine on inflammatory response of IPEC-J2 cells infected with Transmissible gastroenteritis virus (TGEV) of swine[D]. Ya'an:Sichuan Agricultural University, 2019.(in Chinese)
[23] 徐卫华, 高胜东.缺氧诱导因子1α调控claudin-1蛋白表达对肠道粘膜屏障功能的影响[J].湖北民族学院学报(医学版)2016, 33(2):6-9. XU W H, GAO S D.Human hypoxia-inducible factor 1α suppresses claudin-1 expression and impairs intestinal epithelial barrier function[J].Journal of Hubei University for Nationalities (Medical Edition), 2016, 33(2):6-9.(in Chinese)
[24] 郑海军, 邱翠婷, 晋辉, 等.白藜芦醇通过下调缺氧诱导因子1α(HIF-1α)减少小鼠RAW264.7巨噬细胞的凋亡[J].细胞与分子免疫学杂志, 2020, 36(1):42-48. ZHANG H J, QIU C T, JIN H, et al.Resveratrol reduces apoptosis of mouse RAW264.7 macrophage via down-regulating HIF-1α[J].Chinese Journal of Cellular and Molecular Immunology, 2020, 36(1):42-48.(in Chinese)

Exploration on the Mechanism of Modified Wumei Powder in the Treatment of Porcine Transmissible Gastroenteritis Based on Network Pharmacology

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